潍坊萝卜红心病病原鉴定

 本文用生物学、血清学和分子生物学证据证明,引起潍坊萝卜红心病的病原为芜菁花叶病毒(Turnip mosaic virus,TuMV).该病毒可系统侵染曼陀罗、油菜、咸阳黄瓜、丝瓜、大白菜和普通烟,局部侵染苋色藜和假酸浆,不侵染豌豆.病毒粒体弯曲线状,长约700 nm,可由蚜虫传播,在红心病组织内形成风轮状和片层凝集状内含体,它在SDS-琼脂糖凝胶免疫双扩散试验中可与TuMV的抗血清形成明显的沉淀线.该病毒的CP基因共867个核苷酸,编码288个氨基酸,分子量为32.98 kD.该序列与国内外20个TuMV分离物的CP氨基酸序列比较结果表明,这些分离物可以分为5组,其中引起萝卜红心病的病毒与日本的H1J、KYD8lJ、意大利的ITA7同属一组.

Identification of virus infecting radish in Weifang City

Red heart of radish was proved to be a viral disease,and the isolate of samples was identified to be Turnip mosaic virus (TuMV) with evidences at biological,serological and molecular levels. The virus isolate could produce systemic mosaic symptom on Datura metel,Brassica campestris,Cucumis sativus cv. Xianyang, Luffa acutangula,Brassica campestris ssp. pekinensis, and Nicotiana tabacum, local necrotic lesions on Chenopodium amaranticolor, local chlorotic lesions on Nicandra physaloides. It could not infect Pisum sativum. The virus particles were flexuous filamental,with an average length of 700 nm. It produced pin-wheel inclusion bodies and laminated aggregates in infected radish root tissue. The virus isolate could react with antibody to TuMV in SDS-agarose gel double-diffusion test. The coat protein gene contained 867 nucleotides and en- coded 288 amino acids,with molecular weight of 32.98 kD. The phylogenetic analysis result of this isolate and other 20 TuMV isolates indicated that the virus shared higher homology with two Japanese isolates (H1J and KYD81J) and one Italian isolate (ITA7).