| 铁棒锤组织培养技术研究 |
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| 引用本文： | 杜弢,陈红刚,高素芳,张延红,王惠珍,林丽.铁棒锤组织培养技术研究[J].甘肃农业大学学报,2011,46(2):83-86. |
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| 作者姓名： | 杜弢 陈红刚 高素芳 张延红 王惠珍 林丽 |
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| 作者单位： | 甘肃中医学院药学系,甘肃,兰州,730000 |
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| 摘 要： | 以铁棒锤越冬芽为外植体,在添加抗褐化剂聚乙烯吡咯烷酮(PVP)和活性碳(AC)的MS培养上,附加不同浓度组合的NAA和6-BA,进行了芽增殖和试管苗生根培养.结果表明:在MS培养基上添加1.5 g·L-1AC+1.0 g·L-1PVP可抑制培养物褐化;在MS培养基上添加2.5 mg·L-16-BA+0.1 mg·L-1...
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| 关 键 词： | 铁棒锤 越冬芽 组织培养 抗褐化剂 |
Technology for tissue culture of Aconitum pendulum Busch |
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| DU Tao,CHEN Hong-gang,GAO Su-fang,ZHANG Yan-hong,WANG Hui-zhen,LIN Li.Technology for tissue culture of Aconitum pendulum Busch[J].Journal of Gansu Agricultural University,2011,46(2):83-86. |
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| Authors: | DU Tao CHEN Hong-gang GAO Su-fang ZHANG Yan-hong WANG Hui-zhen LIN Li |
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| Abstract: | The hibernaculum of Aconitum pendulum was used as explant,anti-browning agents(PVP and AC) and different concentrations of hormones(NAA and 6-BA) were added in MS culture medium for bud proliferation and rooting.The results showed that 1.0 g·L-1 PVP and 1.5 g·L-1 AC could inhibit browning.MS culture medium added 2.5 mg·L-1 6-BA and 0.1 mg·L-1NAA could induce bud proliferation.1/2 MS culture medium added 0.2 mg·L-1 NAA was benefit for rooting. |
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| Keywords: | Aconitum pendulum Bush hibernaculum tissue culture anti-browning agent |
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