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| 黄瓜绿斑驳花叶病毒单克隆抗体的研制 | |
| 引用本文： | 李桂芬,曹振,马杰,李明福,陈笑瑜,丁建云,李娜. 黄瓜绿斑驳花叶病毒单克隆抗体的研制[J]. 植物保护, 2009, 35(6): 40-42. DOI: 10.3969/j.issn.0529-1542.2009.06.008 |
| 作者姓名： | 李桂芬 曹振 马杰 李明福 陈笑瑜 丁建云 李娜 |
| 作者单位： | 1. 中国检验检疫科学研究院动植物检疫研究所,北京,100024 2. 农业部兽医诊断中心,北京,100193 3. 北京市植物保护站,北京,100029 |
| 基金项目： | 北京财政2006年黄瓜绿斑驳花叶病毒疫情防治控制项目 |
| 摘 要： | 将纯化的黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus, CGMMV)制剂免疫BALB/c小鼠，最后一次免疫后第3天取其脾细胞与SP2/0细胞融合，经采用选择性培养基、有限稀释法克隆和间接ELISA方法进行筛选，成功获得了3株分泌CGMMV特异性单克隆抗体的杂交瘤细胞株并分别命名为3C9,3F7,2G10。用ELISA方法对所获得的3个杂交瘤细胞株进行亚型鉴定均为IgG2a,kappa链。 间接ELISA效价测定结果分别为3C9：1.024×107，3F7：2.56×106，2G10：1.28×106。此3株杂交瘤细胞所分泌的单克隆抗体均能与本研究室保存的其他3种不同的CGMMV分离物发生特异性反应，而不与其他3种同属成员病毒 烟草花叶病毒(Tobacco mosaic virus, TMV)、辣椒轻斑驳病毒(Pepper mild mottle virus, PMMoV)、齿瓣兰环斑病毒(Odontoglossum ringspot virus,ORSV) 发生反应。 |
| 关 键 词： | 黄瓜绿斑驳花叶病毒 单克隆抗体 |
Preparation of the monoclonal antibody against the Cucumber green mottle mosaic virus |
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| Li Guifen,Cao Zhen,Ma Jie,Li Mingfu,Chen Xiaoyu,Ding Jianyun,Li Na. Preparation of the monoclonal antibody against the Cucumber green mottle mosaic virus[J]. Plant Protection, 2009, 35(6): 40-42. DOI: 10.3969/j.issn.0529-1542.2009.06.008 | |
| Authors: | Li Guifen Cao Zhen Ma Jie Li Mingfu Chen Xiaoyu Ding Jianyun Li Na |
| Affiliation: | 1. Institute of Animal and Plant Quarantine, Chinese Academy of Inspection &; Quarantine, Beijing100024, China;2. National Veterinary Diagnostic Center, Ministry of Agriculture, Beijing100193, China;3. Beijing Plant Protection Station, Beijing100029, China |
| Abstract: | The Cucumber green mottle mosaic virus(CGMMV)was injected to BALB/c mice whose cells were fused with myeloma cells SP2/0 3 days after the final injection.On the selective medium,3 hybridoma cell lines were obtained by the finite dilution and indirect-ELISA.The 3 lines can secrete monoclonal antibody(McAbs)stably and were named as 3C9,3F7 and 2G10.respectively.The subtypes were identified by ELISA as IgG2a,Kappa light chain;the titration was examined,which were 3C9:1.024×10~7,3F7:2.56×10~6 and 2G10:1.28×10~6,respectively.By indirect-ELISA,3C9,3F7 and 2G10 were demonstrated to react specifically to the three different CGMMV isolates from other origins,whereas 3C9,3F7 and 2G10 did not response to either of the members of the same genus,TMV,PMMV or ORSV. |
| Keywords: | Cucumber green mottle mosaic virus monoclonal antibody |
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