Activation specificity of commonly employed mitogens for canine B- and T-lymphocytes |
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Authors: | S Krakowka S S Ringler |
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Institution: | 1. Exercise Biochemistry Laboratory, Department of Exercise Science and Health Promotion, Florida Atlantic University, Boca Raton, FL 33431, United States;2. Department of Kinesiology and Health Sciences, Virginia Commonwealth University, Richmond, VA 23284, United States;3. Department of Kinesiology & Sport Management, Texas Tech University, Lubbock, TX 79409, United States |
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Abstract: | Six day in vitro cultures of canine mononuclear leukocytes with or without prior carbonyl iron depletion were established with 5 commonly employed mitogens. Cultures were assessed for B-cell differentiation by induction of cytoplasmic and supernatant released IgG, M and A and T-cell differentiation by expression of Thy-1 antigen on the cell surface of lymphoblasts. The mitogen concanavalin A was shown to be a restricted T-cell mitogen, whereas pokeweed mitogen and protein A from Staphylococcus aureus stimulated maximal B-cell differentiation. These data establish the mitogenic specificity for canine lymphocytes in unfractionated peripheral blood leukocyte cultures and will permit an in vitro evaluation of various substances upon T- and B-lymphocyte functions. |
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