| Identification of FXPRLamide Family Neuropeptides from the Japanese Oak Silkworm, Antheraea yamamai Using Immunocytochemistry Methods |
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| 引用本文： | WEI Zhao-jun LAI Juan-hong ZHAO Yuan. Identification of FXPRLamide Family Neuropeptides from the Japanese Oak Silkworm, Antheraea yamamai Using Immunocytochemistry Methods[J]. 中国农业科学(英文版), 2006, 5(12): 944-951. DOI: 10.1016/S1671-2927(07)60009-1 |
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| 作者姓名： | WEI Zhao-jun LAI Juan-hong ZHAO Yuan |
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| 作者单位： | [1]Department of Biotechnology, Hefei University of Technology, Hefei 230009, P.R.China [2]The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, P.R.China |
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| 摘 要： | In the present study, zooblooting, ELISA, and whole-mount immunocytochemistry methods were used to identify the FXPRLamide family neuropeptides from the Japanese oak silkworm, Antheraea yamamai. The results showed that the genomic DNA from A. yamamai showed positive bands after being hybridized with the fragment of DH-PBAN cDNA from Samia cynthia ricini, which was labeled with [α-32p]-dCTP. The SG showed highest FXPRLamide peptides titer in neural organs. Using an antiserum against Helicoverpa armigera PBAN, PBAN-like immunoreactivity was detected in the SG and TG of A. yamamai by whole-mount immunocytochemistry, and there were three cluster cells in the SG which shows positive PBAN-like immunoreactivity. The titers of FXPRLamide peptides immunoreactivity in the hemolymph were kept at a steady level. During pupation, the titer was increased promptly, but then decreased to a low level after the early pupal stage. The above-mentioned results demonstrate the existence of FXPRLamide family peptides in A. yamamai, but its function needs to be further investigated in the future.
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| 关 键 词： | 天蚕 FXPRLamide家族 神经肽 免疫细胞化学 ELISA |
Identification of FXPRLamide Family Neuropeptides from the Japanese Oak Silkworm, Antheraea yamamai Using Immunocytochemistry Methods |
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| WEI Zhao-jun,LAI Juan-hong,ZHAO Yuan. Identification of FXPRLamide Family Neuropeptides from the Japanese Oak Silkworm, Antheraea yamamai Using Immunocytochemistry Methods[J]. 《Agricultural Sciences in China》, 2006, 5(12): 944-951. DOI: 10.1016/S1671-2927(07)60009-1 |
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| Authors: | WEI Zhao-jun LAI Juan-hong ZHAO Yuan |
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| Affiliation: | 1. Department of Biotechnology, Hefei University of Technology, Hefei 230009, P.R. China
2. The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, P.R. China |
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| Abstract: | In the present study, zooblooting, ELISA, and whole-mount immunocytochemistry methods were used to identify the FXPRLamide family neuropeptides from the Japanese oak silkworm, Antheraea yamamai. The results showed that the genomic DNA from A. yamamai showed positive bands after being hybridized with the fragment of DH-PBAN cDNA from Samia cynthia ricini, which was labeled with [-32P]-dCTP. The SG showed highest FXPRLamide peptides titer in neural organs. Using an antiserum against Helicoverpa armigera PBAN, PBAN-like immunoreactivity was detected in the SG and TG of A. yamamai by whole-mount immunocytochemistry, and there were three cluster cells in the SG which shows positive PBAN-like immunoreactivity. The titers of FXPRLamide peptides immunoreactivity in the hemolymph were kept at a steady level. During pupation, the titer was increased promptly, but then decreased to a low level after the early pupal stage. The above-mentioned results demonstrate the existence of FXPRLamide family peptides in A. yamamai, but its function needs to be further investigated in the future. |
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| Keywords: | Antheraea yamamai FXPRLamide family neuropeptides immunocytochemistry identification |
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