内生枯草芽孢杆菌E1R-J对萝卜、白菜促生作用

通过萝卜、白菜的种子萌发试验、幼苗盆栽试验以及小区试验,对小麦内生枯草芽孢杆菌E1R-J菌株的促生作用进行了研究.结果表明:E1R-J无菌滤液不同浓度稀释液及菌悬液可促进白菜种子的萌发,使萌芽整齐、萌发率增高.其无菌滤液10倍稀释液浇灌处理的盆栽白菜幼苗,株高、鲜质量、干质量分别比清水对照增长53%、200%和700%.同时发现,浇灌处理的盆栽萝卜幼苗与清水对照相比,株高、鲜质量、干质量也分别增长24.4%、215%和159%.田间小区试验结果也证明内生枯草芽孢杆菌E1R-J具有促生作用,但促生效果低于盆栽试验.利用丙酮直接浸提法测定盆栽白菜叶片中的叶绿素含量,发现无菌滤液10倍稀释液和菌悬液10倍稀释液处理的叶绿素含量增高2倍左右.

The Growth-promoting Effect of Endophytic Bacteria E1R-J on Radish and Cabbage

Effect of entophytic bacteria strain E1R-J on cabbage and radish seed germination, seedling growth was studied in plates, pot and outdoor field experiment. The results showed that the cell-free filtrate of strain E1R-J in different dilution and cell suspension promoted cabbage seed germination. In pot experiment, compared with untreated control, cabbage seedling height, fresh weight, dry weight increased 53% ,200% ,700% , and radish seedling height, fresh weight, dry weight increased 24. 4%, 215%, 159% after treated with 10 ×diluted E1R-J cell-free filtrate. The promotion of seedling growth by strain E1R-J was also found in the outdoor plot experiments. The chlorophyll content in the functional Leaves of the cabbage seedlings greatly increased in treatment of strain E1R-J 10×diluted cell-free filtrate and cell suspension of 10~7 cfu/mL compared with untreated control.