猫尾木挥发油对美洲斑潜蝇产卵驱避作用研究

采用室内生物测定和电生理学的方法研究了猫尾木挥发油对美洲斑潜蝇产卵驱避作用以及美洲斑潜蝇对该挥发油的行为反应和触角电位活性.生物测定结果表明,每棵豆苗施用1～5 μL挥发油,大部分处理的落卵量明显低于对照,其中5 μL剂量效果最好,干扰作用指数(IIPC)和驱避率分别为0.289、55.14%.这表明挥发油对美洲斑潜蝇具有明显的产卵驱避作用.四臂嗅觉仪测试中对挥发油的第一选择率明显较低,处理的停留时间大部分低于对照,进入次数仅在250 mL/min流量时处理明显降低.雌雄虫对挥发油的嗅觉反应无差异.美洲斑潜蝇对挥发油表现明显的触角电位活性,且随着挥发油浓度增大而增大.触角电位活性与挥发油量之间的关系符合模型Y=43.414e0.369 1X.应用GC-MS分离出猫尾木挥发油主要含有 21种化合物,其中邻苯二甲酸二丁酯含量最高,占56.55%,其次为邻苯二甲酸二乙基己酯和1-壬烯-3-醇,分别占9.8%和6.64%.

Deterrent Effect of Essential Oil Extracted from Dolichandrone cauda-felina on Oviposition of Liriomyza sativae Blanchard (Diptera: Agromyzidae)

Oviposition-repelling activity of essential oils of Dolichandrone cauda-felina (Hance) Benth. & Hook. F., behavioral tests using four-armed olfactometer and elecroantennograms (EAG) responses using EAG recording technique in Liriomyza sativae, were all studied. The bioassay results showed that the repellent effect of the volatile was dose-dependent;the best effect was at dosage of 5 μL, IIPC and repellent rate were 0.289, 55.14% respectively. Olfactory response of L. sativae indicated the strong repellent effect of the essential oil, and no patterns differences between male and female. The EAG recording produced analogous results: the essential oil elicited an EAG response in leafminers adults, and showed a dose dependent. GC/MS analysis of essential oil indicated that there were 21 known compounds;the dibutyl phthalate had the highest relative concentration (56.55%).