茭白lea3基因全长cDNA的分离克隆及序列分析

第3组胚胎晚期丰富蛋白(LEA3)是一组在胚胎发育后期丰富表达的蛋白,同时它还受水分胁迫和ABA的诱导,与植物抗旱性密切相关。本研究以经过干旱处理的茭白幼苗的mRNA为模板,根据禾本科lea3基因的保守序列设计简并引物进行RT-PCR,得到lea3基因的部分序列,然后结合RACE方法获得全基因两端的序列,经拼接得出茭白lea3基因的全长cDNA,序列分析结果表明该基因的开放阅读框为666bp,编码221个氨基酸,包含2个LEA3基元序列。通过与已报道的LEA3蛋白序列比较,与禾本科中水稻、大麦、小麦、雀麦、玉米的同源性分别为51.2%、41.0%、41.1%、42.0%和40.5%。最终得出结论,茭白lea3基因具有已知物种lea3基因的特征,可以为该基因的功能研究提供参考。目前,该基因已在GenBank中登录,编号为GQ494017。

Segregate Cloning and Sequence Analysis of lea3 Gene Complete cDNA in Zizania caduciflora

Group 3 later embryogenesis abundant proteins(LEA3),present in abundance in seeds during the late stages of development,induced by exogenous abscisic acid(ABA),and associated with drought tolerance.According to high conservative amino acids of reported LEA3 in Gramineae,degenerate primers were designed and a special DNA fragment was obtained from Zizania caduciflora by RT-PCR.Then based on the obtained sequence,some new primers were designed,and two terminals of lea3 gene were cloned by 3'RACE and 5'RACE re...