施肥、刈割对短尖苔草生理生化特征的影响

短尖苔草(Carex brevicuspis)是洞庭湖分布面积最广的植物之一,具有生物量大、营养价值高等特点,是优良的畜牧饲料资源。为探明该植物对施肥、刈割的生理响应机制,从而为开发该饲料植物提供依据,研究了该植物在不同施肥量与刈割次数条件下的生理生化变化。结果表明:随着施肥量的增加,短尖苔草可溶性蛋白含量呈现先下降后上升趋势,较对照增加140.01%;叶绿素、可溶性糖、脯氨酸、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均呈现先上升后下降的趋势。随着刈割次数的增加,短尖苔草叶绿素含量呈现下降的趋势,其降幅达21.30%;SOD活性呈现先上升后下降的趋势,可溶性蛋白含量呈现先下降后上升趋势,POD、CAT活性则呈现上升趋势。可见施肥量为20 g/m2时可促进短尖苔草光合作用,增加抗氧化酶的活性;刈割两次促进了渗透调节物质的合成,为植物恢复生长提供了生理基础。

Effects of fertilization and clipping on the biochemical characteristics of Carex brevicuspis

Carex brevicuspis is one of the most widely distributed plants in Dongting Lake. It has large biomassand high nutritional value, and is an excellent animal husbandry feed resource. In order to reveal the physiologicalresponse mechanism of the plant under different fertilization and clipping intensity, and thus to provide basis for thedevelopment of the feed plant, the physiological and biochemical changes of the plant under different fertilizationand clipping conditions were studied. The results show: With the increasing of fertilization concentration, the solubleprotein content of C. brevicuspis decreased first and then increased, and the highest content increased by 140.01%;All of the contents of chlorophyll, soluble sugar, proline, superoxide dismutase (SOD), peroxidase (POD), catalase(CAT), malondialdehyde (MDA) increased first and then decreased. With the increasing of the clipping frequency,the chlorophyll content of C. brevicuspis decreased by 21.30%. SOD showed a trend of increasing first and thendecreasing, soluble protein decreased first and then increased, and POD and CAT increased. The results illustratedthat fertilization intensity of 20 g/m2 can promote thee photosynthesis of C. brevicuspis, and increased the activity ofthe enzyme; Slipping frequency of two times can promote synthesis of osmolytes, and offered the physiological basis forrecovering growth of C. brevicuspis.