4种核酸染料在实验教学中的应用研究

分别使用4种核酸染料对凝胶中质粒DNA进行染色,紫外凝胶成像仪下观察和记录结果,比较4种核酸染料对琼脂糖凝胶中DNA的染色效果,并对结果进行比较分析。结果表明,EB价格低廉,对含量为50ng以上的DNA着色效果最好,亮度较暗;而Gold view与EB相比染色效果无太大差别,但是亮度比EB稍亮;Sybrgreen在这4种核酸染料中亮度很明显,且对含量为10ng以上的DNA就有荧光呈现;Ultra power对含量为5ng以上的DNA染色效果明显,亮度最亮。由于EB有强致突变性,微毒,而后3种核酸染料基本无毒性,安全可靠,因此,本着经济、方便、可靠的原则,在实验室中用Gold view足以满足一般需要;经济允许且在做荧光定量PCR、即时PCR时,最好选用Sybr green。

Application of Four Nucleic Acid Dyes in Experiment Instruction

DNA of plasmid was stained with four nucleic acid dyes.The staining results were investigated under UV transilluminator.The results show that,the EB price is low,the content is the best DNA coloring effect than 50NG, brightness is dark;and no significant difference between Gold view and EB dyeing effect,but a bit brighter brighter than EB;Sybr green in these four kinds of nucleic acid dye brightness obviously,and there are fluorescent presenta?tion of the content of more than 10NG DNA;Ultra power on the content of 5ng or DNA staining effect is obvious,the most bright brightness.And because EB has strong mutagenicity,micro toxic;the latter three nucleic acid dye is non?toxic,safe and reliable.To sum up,in line with economic,convenient,reliable principle,in the laboratory using Gold view is sufficient to meet the general needs;and in the economy allows fluorescence Quantitative PCR,real-time PCR,the best selection of Sybr green.