鸡新城疫活疫苗室温保存工艺的初步研究

选用海藻糖、明胶、PVP等成分配制耐热保护剂,采用梯度真空干燥方法,将鸡新城疫病毒(La Sota株)进行泡沫干燥,通过37℃10 d耐老化试验筛选到配方T5的病毒滴度损失为0.2 Lg;NDV-T5泡沫干燥疫苗经DSC测定配方玻璃化转变温度Tg为56.45℃,扫描电镜观察可见较为规则的片状结构;NDV-T5在各温度耐热性能均较好,2℃~8℃保存24个月,25℃保存15个月、37℃保存5个月,病毒含量下降≤1.0 Lg;将不同保存温度的NDV-T5泡沫干燥疫苗免疫1月龄雏鸡,鸡只均能在免疫后14 d产生抗体,与现有商品疫苗效力相当。

Study on Room Temperature Preservation Technology of Live Newcasle Disease Virus

The vaccine of newcasle disease virus was prepared by vaccum foam drying. In the process, thermostable protectant was a mixture of trehalose, gelatin, polyvinyl pyrrolidone K30 (PVP) and inorganic water soluble phosphates. The virus titer of formulation T5, lossed only 0.2 Lg at 37℃ for 10 d. The foam vaccine had favorable heat tolerance, could be storaged at 25℃ for 15 months, 37℃ for 5 months, and 2～8℃ for 24 months. The glass-transition temperature of foam vaccine was detected by DSC, showed superordinary temperature of 56.45℃. A regular lamellar structure, the foam vaccine microappearance, was observed under scanning electron microscope (SEM). Compared with commercial vaccine，the HI titer of the foam vaccine has an identical level after immuned for 14 d on chickens.