广西马尾松天然林古蓬和浪水种源群体遗传多样性ISSR分析

从100个ISSR引物中筛选出10个多态性引物,对广西古蓬和浪水两个种源区的马尾松天然林5个群体共85份样品进行遗传多样性分析,古蓬种源三个群体47份样品共检测到192个位点,其中多态位点数为177个,浪水两个群体共38个样品检测到168个多态位点.对两个种源区的马尾松群体ISSR分析数据采用POPgene 32软件进行分析,结果表明:古蓬马尾松天然林群体内,总的多态位点百分率(P)为94.35%.总的Shannon信息指数(I)为0.4278,Nei's基因多样度指数(h)为0.2765;浪水种源马尾松群体内,总的多态位点百分率(P)为94.38%,总的Shannon信息指数(I)为0.5190,Nei's基因多样度指数(h)为0.3510;这表明马尾松天然林群体内遗传多样性水平高,个体闻基因多样性变异大.古蓬马尾松群体间的基因分化系数(Gst)为0.0481,浪水的为0.0363,这表明分别有95.19%和96.37%的变异存在予群体内;古蓬3个群体的基因流为9.8877,浪水两个群体的基因流为13.2643,这表明本研究分析中的种源内群体间存在比较高的近亲交配现象,因此有必要加大对马尾松天然林的保护力度,扩大天然林的保护范围.

ISSR Analysis of Genetic Diversity of Pinnus massoniana on Gupeng and Langshui Nature Populations in Guangxi

Genetic diversity on five nature populations of Pinus massoniana Lamb. in Gupeng and Langshui, Guangxi province, was analyzed by 10 inter simple sequence repeat (ISSR) markers selected from 100 primers. There are 47 individuals within 3 groups in Gupeng and 38 individuals in Langshui population. The results were as follows: (1) There are 177 polymorphic loci among 192 loci in Gupeng and 177 polymorphic loci in Langshui population. (2)The propotion of polymorphic loci (P), the Shannon's diversity indexs(I) and the Nei's diversity indexs(h) were 94.35%, 0.4278 and 0.2765 respectively in Gupeng population. Accordingly the value in Langshui population were 94.38%, 0.5190 and 0.3510 respectively. The low coefficient of genetic differentia-tion (Gst=0.0481 and Gst =0.0363)meant that the majority of genetic variations (95.19 % and 96.37%) occurred within populations.(3) The gene flow within Gupeng groups was 9.8877and that of Langshui groups was 13.2643, which means that high degrees of inbreedings esisted within the two populations respectively. So we concluded that it was necessary to increase the protection of natural populations and to expand the scope of protection of natural populations on Masson's Pine in Guangxi.