黄芪复方剂提取物总黄酮对四氯化碳致大鼠肝损伤保护作用研究

目的:探讨黄芪复方剂提取物总黄酮(EFA)对CCl4致大鼠肝氧化损伤的保护作用及机制。方法:将雄性SD大鼠60只随机分成4组,即灌胃灭菌生理盐水空白对照组和模型组、联苯双酯(100 mg/kg)溶液阳性药物对照组、黄芪复方剂提取物总黄酮(200 mg/kg)组。除空白对照组腹腔注射等量生理盐水外,其余各组腹腔注射四氯化碳玉米油(2 mL/kg)造成大鼠肝氧化损伤模型,后分别于12、24 h将相应大鼠处死,检测血清中ALT、AST、ALB含量与肝组织中MDA、SOD、GSH-PX活性。结果:与模型组比较,经黄酮药物组预防性治疗后,AST、ALT转氨酶明显降低(P<0.05),而ALB含量增加不明显;同时肝脏中MDA含量明显降低(P<0.05),SOD、GSH-PX活性接近空白组,且明显高于模型组(P<0.01)。结论:EFA对四氯化碳造成的大鼠肝氧化损伤具有保护作用。

Protective effect of flavonoid extract from Radix Astragali compound preparation on liver injury induced by carbon tetrachloride

Objective： To investigate the effect and mechaism of flavo.oid sact from Radi Astragali bifendate （100 mg/kg） by gastrogavage twice daily, respectively. After 12 and 24 hours, the contents of ALT, AST and ALB in serum were determined, and the level of hepatic MDA, SOD and GSH-Px were also detected. Results： After treatment with flavonoid extract, the contents of ALT, AST and hepatic MDA in rats were significantly decreased（P〈0.05）, while the contents of ALB had no changes compared with that of the model group. Moreover, the level of SOD and GSH-Px in treatment group were all approximated to that of the normal control, but higher than that of the model group. Conclusion： Flavonoid extract from Radix Astragali compound preparation could protect rats from acute hepatic injury induced by CC14.