| 矮牡丹子叶节离体再生体系 |
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| 引用本文： | 贾文庆,刘会超,姚连芳. 矮牡丹子叶节离体再生体系[J]. 东北林业大学学报, 2010, 38(2) |
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| 作者姓名： | 贾文庆 刘会超 姚连芳 |
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| 作者单位： | 河南科技学院,新乡,453003;河南科技学院,新乡,453003;河南科技学院,新乡,453003 |
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| 基金项目： | 河南省创新人才工程(2005-126-49);;河南科技学院博士基金(050106);;河南科技学院重点项目基金(050121) |
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| 摘 要： | 以矮牡丹子叶作为外植体,研究了不同培养基和植物生长调节剂对其再生体系的影响。结果表明:1/2MS培养基有利于子叶不定芽的分化;在附加6-BA3.0mg·mL-1和NAA0.3mg·mL-1的1/2MS培养基上,每个外植体不定芽的分化率达5.22个;将再生芽转接到含有IBA1.5mg·mL-1和NAA0.5mg·mL-1的1/2MS生根培养基上,生根率达54.17%,生根数达3.12条。
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| 关 键 词： | 矮牡丹 子叶 丛生芽 生根 |
Establishment of in Vitro Regeneration System for Cotyledonary Nodes of Dwarf Peony |
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| Jia Wenqing,Liu Huichao,Yao Lianfang. Establishment of in Vitro Regeneration System for Cotyledonary Nodes of Dwarf Peony[J]. Journal of Northeast Forestry University, 2010, 38(2) |
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| Authors: | Jia Wenqing Liu Huichao Yao Lianfang |
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| Affiliation: | College of Forestry;Henan Institute of Science and Technology;Xinxiang 453003;P.R.China |
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| Abstract: | An experiment was conducted to study the effects of culture medium and plant growth regulators on shoot regeneration from cotyledon of dwarf peony (Paeonia suffruticosa Andr. var. spontanea Rehd.). Results show that 1/2MS medium is suitable for differentiation of adventitious buds. Shoot number reached 5.22 individuals per explant on MS medium supplemented with 3.0mg·L~(-1) 6-BA and 0.3mg·L~(-1) NAA. The rooting rate and root number reached 54.17% and 3.12 after transferred to 1/2MS medium with 1.5mg·L~(-1) IBA and 0.5mg·L~(-1) NAA. |
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| Keywords: | Dwarf peony Cotyledon Multiple buds Rooting |
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