| 鸡贫血病毒衣壳蛋白基因的PCR扩增和克隆 |
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| 引用本文： | 刘岳龙,崔治中,段玉友.鸡贫血病毒衣壳蛋白基因的PCR扩增和克隆[J].扬州大学学报(农业与生命科学版),1997(2). |
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| 作者姓名： | 刘岳龙 崔治中 段玉友 |
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| 作者单位： | 江苏农学院动物医学系 |
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| 摘 要： | 通过聚合酶链式反应（ＰＣＲ）扩增鸡贫血病毒（ＣＡＶ）衣壳蛋白基因（１．４ｋｂ），并将此基因克隆进ｐＵＣ－１８载体中。通过原位杂交、酶切分析、Ｓｏｕｔｈｅｒｎ杂交及其标记成探针对ＣＡＶ基因组的特异性反应等试验证实所获得的克隆是含有ＣＡＶ衣壳蛋白基因的重组质粒克隆。此工作为下一步ＣＡＶ衣壳蛋白体外表达的研究奠定了基础
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| 关 键 词： | 鸡 贫血＋病毒 衣壳蛋白 基因 克隆 |
AMPLIFICATION BY PCR AND CLONING OF THE CAPSID GENE OF CHICKEN ANAEMIA VIRUS |
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| Liu Yuelong,Cui Zhizhong,Duan Yuyou.AMPLIFICATION BY PCR AND CLONING OF THE CAPSID GENE OF CHICKEN ANAEMIA VIRUS[J].Journal of Yangzhou University:Agricultural and Life Science Edition,1997(2). |
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| Authors: | Liu Yuelong Cui Zhizhong Duan Yuyou |
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| Abstract: | The capsid gene (1.4 kb) of chicken anaemia virus (CAV) was amplified by the polymerase chain reaction (PCR) and was cloned into pUC 18. The CAV specific recombinant clone pCC 01 was tested by colony hybridization, restrictive enzymes analysis, Southern hybridization and dot blot with CAV genome DNA after being labelled with Digoxigenin. This work founds for further study on expressing CAV capsid protein in vitro. |
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| Keywords: | domestic fowl anaemia+virus capsid gene clone |
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