Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog |
| |
Authors: | Garner Ethan C Campbell Christopher S Weibel Douglas B Mullins R Dyche |
| |
Affiliation: | Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA. |
| |
Abstract: | Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|