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| 黑暗预处理对蝴蝶兰组培中外植体褐化的影响 | |
| 引用本文： | 赵伶俐,范崇辉,葛红. 黑暗预处理对蝴蝶兰组培中外植体褐化的影响[J]. 西北农业学报, 2006, 15(5): 248-250 |
| 作者姓名： | 赵伶俐 范崇辉 葛红 |
| 作者单位： | 1. 西北农林科技大学,陕西杨凌,712100;中国农业科学院蔬菜花卉研究所,北京,100081 2. 西北农林科技大学,陕西杨凌,712100 3. 中国农业科学院蔬菜花卉研究所,北京,100081 |
| 摘 要： | 以蝴蝶兰R4品种叶片为外植体,研究不同时间的黑暗预处理对褐化率、多酚氧化酶(PPO)活性和总酚含量的影响。结果表明:黑暗预处理能减轻褐化,其中以预处理10 d的褐化最轻;未经暗处理的褐化最重且PPO活性最大,总酚含量无一致变化规律,各处理中褐化率与PPO活性显著相关。 |
| 关 键 词： | 暗培养 蝴蝶兰 组培 褐化 |
| 文章编号： | 1004-1389（2006）05-0248-03 |
| 收稿时间： | 2006-02-20 |
| 修稿时间： | 2006-03-10 |
Effect of Darkness Pretreatment on Browning of Phalaenopsis Explants Cultured in vitro |
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| ZHAO Ling-li,FAN Chong-hui and GE Hong. Effect of Darkness Pretreatment on Browning of Phalaenopsis Explants Cultured in vitro[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2006, 15(5): 248-250 | |
| Authors: | ZHAO Ling-li FAN Chong-hui GE Hong |
| Affiliation: | Northwest A & F University, Yangling Shaanxi 712100, China;Institute of Vegetables and Flowers of Chinese Academy of Agricultural Science, Beijing 100081, China;Northwest A & F University, Yangling Shaanxi 712100, China;Institute of Vegetables and Flowers of Chinese Academy of Agricultural Science, Beijing 100081, China |
| Abstract: | The variety R4 leaf of Phalaenopsis was used as explant,the effect of darkness pretreatment at different time on polyphenol oxidase(PPO) activity,total phenolic content and browning rate in virto were studied.The results as follows: Darkness pretreatment may decrease brown,especially of pretreatment for 10 d,browning rate was the lightest.The activity of PPO was the biggest when explant without darkness pretreatment.Total phenolic content didn't have accordant change rule,there was significant correlation between the browning rate and the activity of PPO in each treatment. |
| Keywords: | Darkness pretreatment Phalaenopsis Tissue culture Browning |
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