岷江百合悬浮细胞系的建立及植株再生

 以岷江百合（Lilium regale Wils.）颗粒细小、结构松散的乳白色愈伤组织为起始材料，接种在MS（NH4NO3含量减半）+ 2.0 mg · L-1 picloram + 30 g · L-1蔗糖液体培养基上，在25 ℃黑暗条件下振荡（100 r · min-1）培养10 ~ 20 d，建立分散性好、均匀、生长迅速的悬浮细胞系。在悬浮细胞系培养过程中研究了不同参数对其生长的影响，结果表明：在40 mL培养液中接种量为0.5 g（鲜样质量），18 d继代1次，继代时保留1/3体积的旧培养液有利于其细胞增殖。将悬浮细胞转移到1/2MS + 30 g · L-1蔗糖的再生培养基上成功获得再生植株。

Establishment of Cell Suspension Culture and Plantlet Regeneration of Lilium regale

Cell suspension culture of Lilium regale Wils. were initiated from creamy-white nodular callus with loose structure. They were cultured in modified liquid MS medium（NH4NO3 with half-strength）supplemented with picloram 2.0 mg · L-1 and sucrose 30 g · L-1，and shook at 100 r · min-1 under dark condition at 25 ℃. About 10–20 days after，stably growing cell suspensioncultures were established. The growth pattern of the cultures was investigated and the optimal parameters were：0.5 g（FW）initial inoculum per 40 mL liquid medium，a sub-culture every 18 days，and 1/3 volume of old liquid medium left during subculture. Suspension cells regenerated plantlets on solid 1/2MS medium with 30 g · L-1 sucrose.