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乙烯和1-MCP的竞争性作用对月季花器官乙烯生成量及相关基因表达的影响
引用本文:薛璟祺,杨丰,左志锐,高俊平.乙烯和1-MCP的竞争性作用对月季花器官乙烯生成量及相关基因表达的影响[J].园艺学报,2011,38(2):311.
作者姓名:薛璟祺  杨丰  左志锐  高俊平
作者单位:(1中国农业科学院蔬菜花卉研究所,北京 100081;2中国农业大学观赏园艺与园林系,北京 100193;3江苏省花卉种质创新工程技术研究中心,江苏苏州 215600)
基金项目:国家自然科学基金项目,农业部园艺作物遗传改良重点开放实验室项目
摘    要: 以探讨月季切花不同花器官的乙烯生物合成和对乙烯的感受为目的,以切花月季‘Samantha’为试材,进行了乙烯和1-MCP交叉处理,测定花朵各器官乙烯生成量,Rh-ACS3、Rh-ACO1和Rh-ETR3基因的表达。结果表明,1-MCP前处理或后处理均能有效抑制花瓣、雌蕊和花托中由于外源乙烯造成的内源乙烯生成量增加。在基因表达方面,Rh-ACS3、Rh-ACO1和Rh-ETR3在花瓣和雌蕊中对乙烯处理的诱导表达均可被1-MCP前处理或后处理有效抑制,而在花托中,这种抑制主要针对Rh-ETR3。这些结果说明,1-MCP对乙烯的竞争性抑制作用主要发生在花瓣、雌蕊和花托上,其竞争效果在乙烯处理前或乙烯处理后均有效。

关 键 词:月季  切花  乙烯  1-MCP  基因表达
收稿时间:2010-11-17

Effect of Ethylene and 1-MCP on Ethylene Production and Related Gene Expression in Floral Tissues of Roses
XUE Jing-qi,Yang Feng,ZUO Zhi-rui,GAO Jun-ping.Effect of Ethylene and 1-MCP on Ethylene Production and Related Gene Expression in Floral Tissues of Roses[J].Acta Horticulturae Sinica,2011,38(2):311.
Authors:XUE Jing-qi  Yang Feng  ZUO Zhi-rui  GAO Jun-ping
Institution:(1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Department of Ornamental Horticulture and Landscape Architecture,Beijing 100193,China;3Engneering Research Center for Floriculture,Suzhou,Jiangsu 215600,China)
Abstract:The effects of ethylene and its inhibitor,1-methylcyclopropene(1-MCP)treatments on the flower opening,ethylene production,and the expression of ethylene biosynthesis- and perception-related genes in cut rose‘Samantha’were investigated. The results showed that 1-MCP pre-treatment or post-treatment substantially inhibited ethylene-caused increase of ethylene production in petals,gynoecia,and receptacles. 1-MCP treatments also substantially inhibited the expression of Rh-ACS3,Rh-ACO1 and Rh-ETR3 genes in petals and gynoecia,but of only Rh-ETR3 in receptacles. The results indicated that the effect of competitive inhibition of 1-MCP on ethylene occurred mainly in petals,gynoecia and receptacles,and the effect was substantial,regardless the treatment was performed before or after ethylene treatment.
Keywords:1-MCP
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