抱茎獐牙菜中3种药效成分的HPLC测定 |
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引用本文: | 马玉花,纪兰菊,陈桂琛,卢学峰.抱茎獐牙菜中3种药效成分的HPLC测定[J].安徽农业科学,2009,37(4):1623-1624. |
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作者姓名: | 马玉花 纪兰菊 陈桂琛 卢学峰 |
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作者单位: | 1. 青海民族学院化学系,青海,西宁,810007 2. 中国科学院西北高原生物研究所,青海,西宁,810001 |
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摘 要: | 目的]测定抱茎獐牙菜中不同部位的药效成分。方法]采用反相高效液相色谱法,测定抱茎獐牙菜中不同部位獐牙菜苦苷、龙胆苦苷及芒果苷的含量。结果]结果表明,HPLC法测定3种药效成分的最佳条件为流动相甲醇-0.02%磷酸水溶液,梯度洗脱,洗脱条件0~25min,甲醇:20%~50%;流速1ml/min,检测波长为260nm。该方法具有很好的线性关系和回收率。结论]抱茎獐牙莱根部龙胆苦苷的含量最高,其余2种药效成分在花的部位有较高的含量。
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关 键 词: | 反相高效液相色谱法 抱茎獐牙菜 獐牙菜苦苷 龙胆苦苷 芒果苷 |
Determination of Three Medicinal Bioactive Components of Swertia franchetiana H.Smith |
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Institution: | MA Yu-hua et al (Department of Chemistry, Qinghai Nationalities University, Xining, Qinghai 810007 ) |
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Abstract: | Objective] The purpose was to determine three medicinal bioactive components in different parts of Swertia franchetiana H.Smith.Method]The content of swertiamarin,gentiopicroside and mangiferin in Swertia franchetiana H.Smith were determined by reversed-phase high performance liquid chromatography.Result]The optimum conditions of determining three medicinal bioactive components in different parts of Swertia franchetiana H.Smith were as follow: a Kromasil C18(250 mm×4.60 mm,5 μm);the mobile phase was composed of CH3OH and 0.02% phosphoric acid in water;the gradient condition was 0-25 min,CH3OH 20%-50%;the flow rate was 1 ml/min;the detection wavelength was 260 nm.The good linear relationships and recoveries were obtained under the optimum conditions.Conclusion]The content of gentiopicroside is most high in the root,swertiamarin and mangiferin have higher content in the floral part of Swertia franchetiana H.Smith. |
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Keywords: | Reversed-phase high performance liquid chromatography Swertia franchetiana H Smith Swertiamarin Gentiopicroside Mangiferin |
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