Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila |
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Authors: | Markert Stephanie Arndt Cordelia Felbeck Horst Becher Dörte Sievert Stefan M Hügler Michael Albrecht Dirk Robidart Julie Bench Shellie Feldman Robert A Hecker Michael Schweder Thomas |
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Affiliation: | Institute of Marine Biotechnology, Walther-Rathenau-Strasse 49, D-17489 Greifswald, Germany. |
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Abstract: | The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has never been successfully cultivated outside its host. In the absence of cultivation data, we have taken a proteomic approach based on the metagenome sequence to study the metabolism of this peculiar microorganism in detail. As one result, we found that three major sulfide oxidation proteins constitute approximately 12% of the total cytosolic proteome, which highlights the essential role of these enzymes for the symbiont's energy metabolism. Unexpectedly, the symbiont uses the reductive tricarboxylic acid cycle in addition to the previously identified Calvin cycle for CO2 fixation. |
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