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| 农杆菌介导多年生黑麦草遗传转化体系的建立 | |
| 引用本文： | 杨茹,袁庆华,曹致中,王锁民. 农杆菌介导多年生黑麦草遗传转化体系的建立[J]. 中国草地学报, 2010, 32(1) |
| 作者姓名： | 杨茹 袁庆华 曹致中 王锁民 |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所,北京,100094;甘肃农业大学草业学院,甘肃,兰州,730070 2. 中国农业科学院北京畜牧兽医研究所,北京,100094 3. 甘肃农业大学草业学院,甘肃,兰州,730070 4. 兰州大学草地农业科技学院,甘肃,兰州,730000 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | AVP1基因能够提高植物的抗盐性,通过农杆菌介导AVP1基因转化多年生黑麦草,并建立了较为成熟的遗传转化体系。研究表明,将预培养4d的黑麦草胚性愈伤组织经OD600为0.6左右的农杆菌菌液在负压下侵染6min,共培养3d,用250mg/L的羧苄青霉素(Carb)脱菌,附加150μmol/L的乙酰丁香酮(AS),然后转接到含75mg/L潮霉素(Hpt)的筛选培养基上进行筛选培养的转化体系效果最佳。 |
| 关 键 词： | 多年生黑麦草 AVP1基因 农杆菌 遗传转化 |
Establishment and Optimization of Perennial Ryegrass Genetic Transformation System with Agrobacterium-mediated Method |
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| YANG Ru,YUAN Qing-hua,CAO Zhi-zhong,WANG Suo-min. Establishment and Optimization of Perennial Ryegrass Genetic Transformation System with Agrobacterium-mediated Method[J]. Chinese Journal of Grassland, 2010, 32(1) | |
| Authors: | YANG Ru YUAN Qing-hua CAO Zhi-zhong WANG Suo-min |
| Affiliation: | YANG Ru1,2,YUAN Qing-hua1,CAO Zhi-zhong2,WANG Suo-min3(1.Institute of Animal Sciences,CAAS,Beijing 100094,China,2.College of Grassl, Science,Gansu Agricultural University,Lanzhou 730070,3.College of Grassl, Science , Technology,Lanzhou University,Lanzhou 730020,China) |
| Abstract: | The perennial ryegrass genetic transformation system with Agrobacterium-mediated Method was studied. The calluses obtained after about 4 days culture were used as recipient material for genetic transformation. The concentration of Agrobacterium tumefaciens suspension was OD_(600)=0.6 and added AS of 150μmol/L. Then the calluses were incubated at 24℃ in the dark of 3 days.After that those calluses were cultivated in the medium supplemented the dark for 3 days. After that those calluses were cultivated in the medium supplemented with 75mg/L Hpt and 250mg/L Carb for selection. |
| Keywords: | Perennial ryegrass AVP1 gene Agrobacterium tumefaciens Genetic transformation |
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