光敏核不育水稻OsRacD启动子的分离及其育性相关性分析

以水稻OsRacD的cDNA序列为探针筛选光敏核不育水稻农垦58S基因组文库,获得了一个包含2 kb的OsRacD启动区和396 bp编码区序列的阳性克隆。与反向PCR克隆的水稻农垦58N该基因的启动区比较，证实该基因的启动区在农垦58S和农垦58N的同源性达到99.8%，且在预测的调控元件上不存在碱基差异。说明农垦58S和农垦58N在长日照条件下表现出的育性差别，并非两者在OsRacD调控序列结构上的差别所致，而与基因表达的发育调控有关。

Isolation and Analysis of Promoter of OsRacD from Photoperiod Sensitive Genic Male Sterile Rice

Using OsRacD eDNA as probe to screen the genomic library of photoperiod sensitive genie male sterile rice Nongken 58S, a positive clone containing 2 kb promoter and 396 bp coding region of OsRacD was obtained. Compared with the promoter of OsRacD cloned by reverse PCR from rice variety Nongken 58N, the homology was 99.8%, and the different nucleotides were outside the predicted response clements in promoter, suggesting thai the fertility difference between Nongken 58S and Nongken 58N under long day conditions was not attributed to the difference in the structure of OsRacD upstream regulation sequences, but to the developmental regulation of gene differential expression.