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| 双肌臀大约克种猪的应激敏感基因净化研究 | |
| 引用本文： | 李建农,何正东,刘红林,陈杰,张建生,顾建平,顾炯.双肌臀大约克种猪的应激敏感基因净化研究[J].畜牧与兽医,2001,33(1):14-15. |
| 作者姓名： | 李建农 何正东 刘红林 陈杰 张建生 顾建平 顾炯 |
| 作者单位： | 1. 江苏省畜牧兽医总站, 2. 南京农业大学动物科技学院 3. 国营常熟畜禽良种场 |
| 基金项目： | 常熟市科委计划项目，1998年度江苏省种猪新品种更新工程项目。 |
| 摘 要： | 应用PCR RFLPs技术对双肌臀大约克 (Ⅰ系 )、普通大约克 (Ⅱ系 )和Ⅰ系与Ⅱ系系间繁殖后代 (Ⅲ系 ) 3个选育群体的全部种猪普检应激敏感基因 (RYR1基因 )。Ⅰ系和Ⅲ系全部为阴性纯合体 ,Ⅱ系检出阳性纯合体和杂合体分别为 1头和 1 5头 ,阳性率为 1 4 2 %。对Ⅱ系的阳性个体和相同数量的阴性个体进行复检 ,其检测结果复重率达 1 0 0 %。经过系统选育 ,培育成了无RYR1基因的双肌臀大约克种猪Ⅰ、Ⅱ和Ⅲ系 1 1 7、 97和 49头的育种核心群。 |
| 关 键 词： | 双肌臀大约克猪 RYR1基因 |
| 文章编号： | 0529-5130(2001)01-0014-02 |
Study on elimination of RYR1 gene from Double Muscled Large White pigs |
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| LI Jian nong ,HE Zheng dong ,LIU Hong lin ,CHEN Jie ,ZHANG Jian sheng ,GU Jian pin ,GU Jiong.Study on elimination of RYR1 gene from Double Muscled Large White pigs[J].Animal Husbandry & Veterinary Medicine,2001,33(1):14-15. | |
| Authors: | LI Jian nong HE Zheng dong LIU Hong lin CHEN Jie ZHANG Jian sheng GU Jian pin GU Jiong |
| Institution: | LI Jian nong 1,HE Zheng dong 1,LIU Hong lin 2,CHEN Jie 2,ZHANG Jian sheng 3,GU Jian pin 3,GU Jiong 3 |
| Abstract: | RYR1 gene was detected by means of PCR-RFLPs for all the bo arsfrom three pig groups,which were Double Muscled Large White pigs(groupⅠ),La rge White pigs(groupⅡ)and offsprings of these two groups(groupⅢ).The result in dicated that all of the Ⅰ and Ⅲ groups were negative homozygotes.However ,there were 1 possitive homozygotes and 15 heterzygotes in the groupⅡ,whose per centage of possitive homozygotes is 14.2%.We assayed all pigs of the groupⅡ tw o times,and the results were completely the same.After being selected systematic ly,we have bred a group of Double Muscled Large White pigs free from RYR1 gene. |
| Keywords: | Double-Muscled Large White pigs RYR1-gene |
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