山东莱州发现三倍体薤白

1材料与方法供试薤白取自山东省莱州市郊外。取薤白的根尖用0．002mol／L 8-羟基喹啉溶液预处理4h，卡诺氏液在冰箱中固定24h，以1mol／L的盐酸60℃解离6～9min，再用改良苯酚品红染色液染色后制片。镜检并统计染色体数目，然后选用染色体形态好且分散的细胞，在配有Olympus DV70数码摄像头的多功能系统显微镜（Olympus BX50）下拍照分析。核型分析按照李懋学、陈瑞阳报道的标准进行，核型分类参照Stebbins GL的方法划分。

Discovery of Triploid Allium macrostemon Bunge in Laizhou City of Shandong Province

[Objective] The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province. [Method] The root tip of A.macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [Result] Allium macrostemon Bunge in Laizhou City introduced in this study was triploid; its somatic chromosome number was 24 and karyotype formula was K(2n)=3x=24m(2SAT)+1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells. [Conclusion] This introduction of triploid A.macrostemon found in China was the first time.