建国以来山东省小麦品种及其亲本Glu-1位点的亚基组成和多样性分析

探讨建国以来山东省小麦品种Glu-1的亚基特点及其多样性，并有针对性地筛选含有优质亚基的亲本。用SDS-PAGE法对山东省建国以来推广的主要品种及其亲本的HMW-GS组成和多样性进行了分析。山东省小麦品种及骨干亲本在Glu-A1位点主要为两种等位变异类型“Null”和“1”；Glu-B1有“7+8”、“7+9”、“14+15”、“17+18”、“6+8”5个等位变异类型，但主要以“7+8”和“7+9”为主；Glu-D1有“2+12”、“2+10”、“5+10”、“4+10”、“4+12”、“2+11”6个等位变异类型，以“2+12”为主。在3个位点中，Glu-B1位点的多样性最丰富，其次为Glu-D1位点，Glu-A1位点的多样性最差。Glu-1的多态性指数从50年代的高点开始下降，在60年代达到最低点，之后缓慢上升，在90年代达到最高点，当前表现为下降的趋势。山东省小麦品种及其亲本缺少优质亚基组合，这为山东省小麦品种的改良提供了方向。

The Composition and Polymorphism Analysis of Glu-1 Subunits of Wheat Varieties and Their Parents in Shandong Province after Liberation

The aim of this paper was to study the composition and polymorphism of Glu-1 Subunits of wheat varieties that selected from liberation to now in Shandong province and select breeding material to improve wheat quality. The Composition and Polymorphism of Glu-1 Subunits were analyzed by SDS-PAGE. The results showed that “null” and “7+9” were the predominant allele variation patterns on Glu-A1 loci. Five allele variation patterns, “7+8”, “7+9”, “14+15”, “17+18” and “6+8” were detected on Glu-B1 loci, but the predominant patterns were “7+8” and “7+9”. Six allele variation patterns, “2+12”, “2+10”, “5+10”, “4+10”, “4+12” and “2+11” were detected on Glu-D1 loci, but the predominant pattern was “2+12”. In those three loci, Glu-B1 loci had the highest polymorphic frequency, Glu-D1 loci was second, and the last was Glu-A1. The polymorphism index of Glu-1 decreased gradually from 1950’ and reached to the lowest point in 1960’. After that the polymorphism index of Glu-1 increased slowly and got the highest point in 1990’. But the index showed decrease from 1990’ to now. The frequencies of the high quality subunits were low in wheat variety and its parent in Shandong province. This could be a way to improve wheat quality of Shandong province.