| 绵羊雌激素受体基因外显子4多态性分析 |
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| 引用本文： | 狄冉,贾立华,储明星,陈宏权,方丽. 绵羊雌激素受体基因外显子4多态性分析[J]. 中国畜牧兽医, 2008, 35(12): 89-92 |
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| 作者姓名： | 狄冉 贾立华 储明星 陈宏权 方丽 |
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| 作者单位： | 1.中国农业科学院北京畜牧兽医研究所，中国农业科学院家养动物遗传资源与种质创新重点开放实验室， 北京 100193；2.安徽农业大学动物科技学院， 合肥 230036 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家高技术研究发展计划(863计划)，国家自然科学基金，北京市自然科学基金，北京市科技计划课题，北京市农委课题，中国农业科学院北京畜牧兽医研究所基本科研业务费专项基金 |
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| 摘 要： | 根据GenBank发表的人、鸡、大鼠雌激素受体(estrogen receptor，ESR)基因外显子4的序列设计1对引物，采用PCR SSCP技术分析ESR基因外显子4在高繁殖力绵羊品种（小尾寒羊和湖羊）和低繁殖力绵羊品种（特克塞尔、中国美利奴、考力代和杜泊）中的单核苷酸多态性，同时研究该基因对小尾寒羊高繁殖力的影响。结果表明,ESR基因的此对引物扩增片段在所检测的6个绵羊品种中均不存在PCR SSCP多态性，说明所检测的ESR基因外显子4序列比较保守，该区域可能不是影响绵羊高繁殖力的功能结构域。
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| 关 键 词： | 绵羊 繁殖力 雌激素受体基因 PCR SSCP |
Polymorphism Analysis of Exon 4 of Estrogen Receptor Gene in Sheep |
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| DI Ran,JIA Li-hua,CHU Ming-xing,CHEN Hong-quan,FANG Li. Polymorphism Analysis of Exon 4 of Estrogen Receptor Gene in Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2008, 35(12): 89-92 |
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| Authors: | DI Ran JIA Li-hua CHU Ming-xing CHEN Hong-quan FANG Li |
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| Affiliation: | 1. The Key Laboratory of Domestic Animal Genetic Resources and Germplasm Innovation of CAAS, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China |
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| Abstract: | The estrogen receptor(ESR) gene was studied as a candidate gene for the high prolificacy of Small Tail Han sheep.A pair of primers was designed by sequence of exon 4 of ESR gene in human,chicken and rat.Single nucleotide polymorphisms(SNPs) of exon 4 of ESR gene were detected in both high prolificacy breeds(Small Tail Han and Hu sheep) and low prolificacy breeds(Texel,Chinese Merino,Corriedale and Dorper sheep) by polymerase chain reaction(PCR)-single strand conformation polymorphism(SSCP).No polymorphism was detected in the amplified region in the six sheep breeds tested.The results indicated that the sequence of exon 4 of ESR gene was rather conserved,and exon 4 may not be the functional domain that affects the high prolificacy in sheep. |
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| Keywords: | sheep prolificacy estrogen receptor gene PCR-SSCP |
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