Assessment of latent infection frequency in progeny tubers of advanced potatoclones resistant to bacterial wilt: A new selection criterion |
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Authors: | Sylvie Priou Clara Salas F De Mendiburu P Aley Liliam Gutarra |
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Institution: | (1) International Potato Centre (CIP), P.O. Box 1558, Lima 12, Peru |
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Abstract: | Summary This paper reports results of a 3-year evaluation of CIP advanced potato clones in a bacterial wilt-infested field (race 3)
in Peru. Clones resistant or moderately resistant to wilt were selected and all tubers harvested from each clone were tested
for latent infection byRalstonia solanacearum using a sensitive serological technique developed at CIP. A sampling strategy to estimate accurately the frequency of infected
tubers in the clones has been evaluated. This method will allow consideration of tuber latent infection as a new selection
criterion in breeding for resistance to bacterial wilt. Thirteen clones were found resistant to wilt in all three evaluations
(i.e.≤6% wilt), from which five had no wilt in all trials. However, all clones harboured latent infection in tubers averaging
30%. Analysing 30 tubers/clone provides an accurate estimation of the proportion of infected tubers with a high precision
level. |
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Keywords: | breeding brown rot disease resistance NCM-ELISA Ralstonia solanacearum sampling serological detection Solanum tuberosum L |
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