| 炎毒热清及其微乳制剂小鼠急性毒性试验和体外抑菌作用(英文) |
| |
| 引用本文： | 王棋文,李剑勇,牛建荣,周旭正,张继瑜,魏小娟,杨亚军,赵兴绪. 炎毒热清及其微乳制剂小鼠急性毒性试验和体外抑菌作用(英文)[J]. 农业科学与技术, 2008, 9(5): 113-117 |
| |
| 作者姓名： | 王棋文 李剑勇 牛建荣 周旭正 张继瑜 魏小娟 杨亚军 赵兴绪 |
| |
| 作者单位： | 中国农业科学院兰州畜牧与兽药研究所新兽药工程重点实验室;甘肃农业大学动物医学院 |
| |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金项目 ， 甘肃省科技支撑计划 |
| |
| 摘 要： | [Objective] The aim of this study is to evaluate the safety and bacteriostasis of Yandureqing(AEE)and its microemulsion(AEE-ME).[Method]The acute toxicity was tested in mice by intragastric administration,and median lethal dose(LD50)as well as its 95% confidence interval was calculated by modified Karber method;the bacteriostasis was investigated by cylinder plate method.[Result]LD50 of AEE in mice was 10.937 g/kg with the 95% confidence interval of 9.309-12.850 g/kg;and LD50 of AEE-ME in mice was 5.357 g/kg with the 95% confidence interval of 4.388-6.566 g/kg.The MICs of AEE to Escherichia coli O149 from swine,Staphylococcus aureus,Salmonella pullorum and Streptococcus agalactiae were 10.00,20.00,20.00 and 10.00 mg/ml,respectively;while the MICs of AEE-ME to the 4 kinds of bacteria mentioned above were 5.00,10.00,5.00 and 5.00 mg/ml in turn,and that to Pseudomonas aeruginosa is 20.00 mg/ml.[Conclusion]AEE is an actually nontoxic drug and AEE-ME belongs to the low toxic preparation.AEE and AEE-ME have obvious bacteriostasis,in which AEE-ME is superior to AEE.
|
| 关 键 词： | Yandureqing Microemulsion Median lethal dose(LD50) Bacteriostasis |
The Acute Toxicity Test in Mice and Bacteriostasis in vitro of Yandureqing and Its Microemulsion |
| |
| WANG Qi-wen,LI Jian-yong,NIU Jian-rong,ZHOU Xu-zheng,ZHANG Ji-yu,WEI Xiao-juan,YANG Ya-jun,ZHAO Xing-xu. The Acute Toxicity Test in Mice and Bacteriostasis in vitro of Yandureqing and Its Microemulsion[J]. Agricultural Science ＆ Technology, 2008, 9(5): 113-117 |
| |
| Authors: | WANG Qi-wen LI Jian-yong NIU Jian-rong ZHOU Xu-zheng ZHANG Ji-yu WEI Xiao-juan YANG Ya-jun ZHAO Xing-xu |
| |
| Affiliation: | 1.Key Laboratory of New Animal Drug Project,Lanzhou Institute of Animal and Veterinary Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050;2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070 |
| |
| Abstract: | [Objective] The aim of this study is to evaluate the safety and bacteriostasis of Yandureqing (AEE) and its microemulsion (AEE-ME). [Method] The acute toxicity was tested in mice by intragastric administration, and median lethal dose (LD50) as well as its 95% confidence interval was calculated by modified Karber method; the bacteriestasis was investigated by cylinder plate method. [Result] LD50 of AEE in mice was 10.937 g/kg with the 95% confidence interval of 9.309-12.850 g/kg; and LD50 of AEE-ME in mice was 5.357 g/kg with the 95% confidence interval of 4. 388-6.566 g/kg. The MICs of AEE to Escherichia coli O149 from swine, Staphylococcus aureus, Salmonella pullorum and Streptococcus agalactiae were 10.00, 20.00, 20.00 and 10.00 mg/ml, respectively; while the MICs of AEE-ME to the 4 kinds of bacteria mentioned above were 5.00, 10.00, 5.00 and 5.00 mg/ml in turn, and that to Pseudomonas aeruginosa is 20.00 mg/ml. [Conclusion] AEE is an actually nontoxic drug and AEE-ME belongs to the low toxic preparation. AEE and AEE-ME have obvious bacteriostasis, in which AEE-ME is superior to AEE. |
| |
| Keywords: | Yandureqing Miercemulsion Median lethal dose (LD50) Bacteriostasis |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
