| 花生体细胞胚发生及植株再生 |
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| 引用本文： | 刘艳芝,韦正乙,谭化,高明.花生体细胞胚发生及植株再生[J].吉林农业科学,2008,33(4). |
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| 作者姓名： | 刘艳芝 韦正乙 谭化 高明 |
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| 作者单位： | 吉林省农业科学院生物技术中心,长春,130033;吉林省农业科学院生物技术中心,长春,130033;吉林省农业科学院生物技术中心,长春,130033;吉林省农业科学院生物技术中心,长春,130033 |
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| 摘 要： | 以花生优良品种四粒红成熟种子胚轴为外植体,诱导体细胞胚发生并获得了再生植株。将胚轴切成小段,接种于附加40 mg/L的MS培养基上诱导胚性愈伤组织,愈伤组织转移至2,4-D 20 mg/L的MS培养基上形成体细胞胚。体细胞胚发生率达到66.67%;体细胞胚在BA浓度逐渐降低的MS培养基上培养,发育成完整植株。
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| 关 键 词： | 花生 愈伤组织 体细胞胚发生 |
Somatic Embryogenesis and Plantlet Regeneration in Peanut |
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| LIU Yan-zhi,WEI Zheng-yi,TAN Hua,GAO Ming.Somatic Embryogenesis and Plantlet Regeneration in Peanut[J].Jilin Agricultural Sciences,2008,33(4). |
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| Authors: | LIU Yan-zhi WEI Zheng-yi TAN Hua GAO Ming |
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| Abstract: | The hypocotyls of mature seed of an eminent cultivar of peanut,'Hongsili' were used for explants for somatic embryogenesis induction and,the regenerated plants were obtained.The hypocotyls were cut into segments and were placed on the MS medium contained 40mg/L 2,4-D to induce callus.The calli were transferred to MS medium with 20 mg/L 2,4-D for somatic embryo formation,the ratio of embryogenesis is 66.67% and the somatic embryos developed into wholly plants on the MS medium with gradually lowered concentration of BA. |
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| Keywords: | Peanut Callus Somatic embryogenesis |
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