| 山奈不同溶剂提取物对DPPH自由基的清除作用 |
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| 引用本文： | 王锐,何嵋,周云,刘忠荣,刘磊. 山奈不同溶剂提取物对DPPH自由基的清除作用[J]. 安徽农业科学, 2010, 38(29): 16232-16233. DOI: 10.3969/j.issn.0517-6611.2010.29.063 |
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| 作者姓名： | 王锐 何嵋 周云 刘忠荣 刘磊 |
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| 作者单位： | 昭通师范高等专科学校化学系,云南昭通,657000;昭通师范高等专科学校化学系,云南昭通,657000;昭通师范高等专科学校化学系,云南昭通,657000;昭通师范高等专科学校化学系,云南昭通,657000;昭通师范高等专科学校化学系,云南昭通,657000 |
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| 基金项目： | 云南省教育厅科学研究基金项目(08C0234) |
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| 摘 要： | [目的]为山奈的综合利用和新型天然抗氧化剂的开发提供理论依据。[方法]山奈干燥粉碎后,用石油醚索氏抽提脱脂,以95%乙醇为溶剂,超声提取得山奈粗提物。粗提物依次用乙酸乙酯、正丁醇和重蒸水萃取,分别得到乙酸乙酯提取物（EAK）、正丁醇提取物（BK）和水提取物（WK）。用清除DPPH自由基法测试山奈不同溶剂提取物的抗氧化活性。[结果]对0.081mg/mlDPPH自由基清除率为50%时,山奈的乙酸乙酯提取物（EAK）、正丁醇提取物（BK）和水提取物（WK）的使用浓度分别为5.48、1.10、10.02mg/ml,3种提取物对自由基的清除能力（DPPH质量/提取物质量）分别为14.78、73.64、8.08。[结论]山奈正丁醇提取物对自由基的清除效果最好。
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| 关 键 词： | 山奈提取物 DPPH 自由基 抗氧化 |
Study on DPPH Free Radical Scavenging Activities of Extracts from Kaempferia galanga L. with Different Sovents |
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| WANG Rui et al. Study on DPPH Free Radical Scavenging Activities of Extracts from Kaempferia galanga L. with Different Sovents[J]. Journal of Anhui Agricultural Sciences, 2010, 38(29): 16232-16233. DOI: 10.3969/j.issn.0517-6611.2010.29.063 |
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| Authors: | WANG Rui et al |
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| Affiliation: | WANG Rui et al(Chemistry Department of Zhaotong Teacher's College,Zhaotong,Yunnan 657000) |
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| Abstract: | [Objective] The study was to provide the theoretical basis for the comprehensive utilization of Kaempferia galanga L.and the development of a new type of natural antioxidant.[Method] The K.galanga was degreased by using petroleum ether after it was dried and crushed,and then the crude extract from it was extracted by ultrasonic with 95% ethanol as solvent.The crude extracts were extracted by ethyl acetate,n-butanol and redistilled water successively.And the ethyl acetate extract(EAK),n-butanol extract(BK)an... |
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| Keywords: | Kaempferia galanga L.extract DPPH Free radicals Antioxidant |
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