87份甘蓝型油菜恢复系与2份优良不育系的遗传差异

利用SSR和SRAP 2种分子标记技术对87份甘蓝型油菜恢复系及2份不育系材料的遗传差异进行研究。2种标记混合聚类分析表明,在相似系数为0.65处将89份材料分成4大类,除恢复系83外,其余恢复系与2份不育系分布在不同类群中,说明这些恢复系与不育系亲缘关系较远,这与材料的遗传背景相一致。另外,在相似系数0.75处87份恢复系又被划分到14个不同亚类中,没有完全聚在一起,说明这些恢复系具有丰富的遗传多样性。遗传距离分析表明,编号为87的不育系与恢复系遗传距离变幅为0.325~0.492,平均遗传距离为0.414;编号为88的不育系与恢复系的遗传距离变幅为0.264~0.492,平均遗传距离为0.398。在以后的育种过程中,可以考虑从中选择分别与2个不育系的遗传距离在前20位的恢复系作为父本,以相应不育系作为母本配置杂交组合,选育优良杂交品种。

Genetic Diversity of Eighty seven Restorer Lines and Two Excellent Male Sterile Lines in Brassica napus

Genetic differences of 87 Brassica napus restorer lines and 2 male sterile lines were studied by using SSR and SRAP molecular markers. The cluster analysis indicated that all the materials were grouped into 4 classes when the similarity coefficient was 0.65. The restorer lines and 2 male sterile lines were distributed in different groups except No. 83 of restorer line. It indicated that these restorer lines and sterile lines had distant genetic relationship, which was consistent with the genetic background of the materials. In addition, when the similarity coefficient was 0.75, the restorer lines were divided into 14 different sub groups without in one group completely, it illustrated that these restorer lines had a rich genetic diversity. The genetic distance of all the materials was analyzed. The results showed that the genetic distance between and restorer lines ranged from 0.325 to 0.492, with the average of 0.414. The genetic distance between No. 88 of sterile line and restorer lines ranged from 0.264 to 0.492, with the average of 0.398. In the subsequent breeding process, we can consider to choose the restorer lines whose genetic distance with 2 male sterile lines was in the top 20 as male parent, with the corresponding male sterile line as female parent to configure hybrid combination and breeding hybrid varieties.