青花菜自交系的遗传多样性及花球营养成分

采用ISSR分子标记技术,对10份多年自交纯化、整齐度较高的青花菜自交系材料(N237、N244、N256、N279、N318、N364、N437、N614、N622、N628)进行遗传多样性及亲缘关系分析,并对自交系花球的营养成分含量进行测定。结果表明:各自交系之间的遗传相似系数为0.557 9~0.852 6,平均相似系数为0.703 2;10份自交系材料聚为二大类(A、B类),其中,A类分为4个亚类;根据花球的维生素C、可溶性蛋白质含量等的测定结果,从10份自交系材料中筛选出了1份维生素C含量高的材料(N256)和3份综合表现较好的材料(N237、N279和N628)。

Analysis of genetic diversity and curd nutrients on broccoli inbred lines

Ten broccoli inbred lines (N237, N244, N256, N279, N318, N364, N437, N614, N622, N628) which are highly homozygous and uniform were used for genetic diversity and genetic relationship analysis based on inter simple sequence repeat (ISSR) molecular markers. Meanwhile, six nutrients of curds in these materials were also tested. Results showed that the overall similarity values among ten inbred lines ranged from 0.557 9 to 0.852 6 with an average of 0.703 2. From the cluster analysis result, ten broccoli inbred lines were divided into two clades (A and B). And clade A was further separated into four sub-clades. One inbred line which was rich in vitamin C (N256) and three inbred lines which had good multiple characters (N237, N279 and N628) were screened.