陕西紫阳县山羊Fas和Bcl-2基因表达研究

为了研究陕西省紫阳县(富硒地区)山羊各组织中Fas和Bcl-2基因表达的情况,探讨微量元素硒对Fas和Bcl-2基因表达的影响。将年龄相仿、体质量相近,分别来自紫阳县双安镇(高硒区,n=7)和高滩镇(低硒区,n=7)的山羊作为研究对象,运用实时荧光定量PCR(qRT-PCR)技术检测心脏、肝脏、脾脏、肺脏、肾脏、肾周脂肪、背最长肌、股二头肌和淋巴组织中Fas和Bcl-2基因mRNA的表达量。结果表明:双安镇山羊各组织Fas基因mRNA表达量由高到低依次为肝脏心脏肺脏肾脏肾周脂肪脾脏股二头肌淋巴背最长肌;高滩镇心脏和肝脏中mRNA的表达量最高,显著高于肾周脂肪、肾脏、背最长肌、股二头肌、肺脏、淋巴、脾脏(P0.05),肝脏、肺脏(P0.01)和肾脏(P0.05)中的表达量均显著低于双安镇。双安镇山羊肺脏中Bcl-2基因mRNA表达量最高,与脾脏和肾周脂肪组织差异不显著(P0.05),但是显著高于其他组织(P0.05);而高滩镇山羊Bcl-2基因mRNA表达量由高到低依次为肾周脂肪肺脏心脏脾脏肾脏淋巴股二头肌肝脏背最长肌,且心脏、脾脏、肾周脂肪(P0.01)和肺脏(P0.05)均高于双安镇山羊。由此可见,Fas和Bcl-2基因mRNA在紫阳县山羊各组织中均有不同程度表达,且微量元素硒上调了山羊组织中Fas基因的表达,抑制Bcl-2基因,但是硒对Fas和Bcl-2基因表达的影响存在组织差异性。

Gene Expression of Fas and Bcl-2 in Goats from Ziyang County of Shaanxi Province

This study was intended to ascertain the mRNA expression levels of Fas and Bcl-2 genes in some tissues of goat from Ziyang County,where is a selenium-rich region in China. Goats with similar age and weight were treated as the research objects,and they came from Shuangan Town (High selenium,n=7) and Gaotan Town (Low selenium,n=7) of Ziyang County,respectively. Real-time quantitative PCR (qRT-PCR) was used to detect the relative mRNA expression levels of Fas and Bcl-2 in heart,liver,spleen,lung,kidney,perirenal fat,longissimus muscle,biceps femoris and lymphatic. The descending order of mRNA expression levels of Fas was liver> heart> lung> kidney> perirenal fat> spleen> biceps femoris> lymphatic> longissimus muscle in the goats from Shuangan Town. In Gaotan Town,the levels of Fas mRNA in heart and liver were higher than that in other tissues (P<0.05),and the mRNA levels in liver,lung (P<0.01) and kidney (P<0.05) were lower than that from Shuangan Town. As to Bcl-2,the mRNA level in lung was the highest,but not higher than that in lymphatic and perirenal fat. What''s more,the descending order of mRNA expression levels of Bcl-2 was perirenal fat> lung> heart> spleen> kidney> lymphatic> biceps femoris> heart> liver> longissimus muscle from Gaotan Town,and the of mRNA levels of Bcl-2 in heart,spleen,perirenal fat (P<0.01) and lung (P<0.05) from Gaotan Town were higher than that from Shuangan Town. Thus Fas and Bcl- 2 genes expressed differently degree and widely in various tissues of goats from Ziyang County,and the expression was affected by trace element selenium but with organizational differences.