| 猪O型FMDV主要抗原表位多肽的合成与鉴定 |
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| 引用本文： | 杨苏珍,赵冬,郅玉宝,邓瑞广,张改平. 猪O型FMDV主要抗原表位多肽的合成与鉴定[J]. 西北农业学报, 2014, 23(10): 8-12 |
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| 作者姓名： | 杨苏珍 赵冬 郅玉宝 邓瑞广 张改平 |
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| 作者单位： | （1.河南省农业科学院 动物免疫学重点实验室,郑州 450002;2.河南农业大学 牧医工程学院,郑州 450002） |
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| 基金项目： | 农业部公益性行业专项（201203039）;河南省农科院科研发展专项资金(20137926)。 |
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| 摘 要： | 为筛选口蹄疫病毒表位抗原,使用Symphony 12通道多肽合成仪,采用Fmoc固相合成原理,合成5条O型FMDV抗原表位肽。Quattro Micro液-质联用仪分析多肽分子的结构特性,分析结果表明,在误差允许的范围内所检测的结果与理论值相符,5条多肽均通过质谱检测。通过SMCC双功能偶联剂将多肽偶联于BSA载体蛋白,以Dot-blot和间接ELISA法检测这5条与BSA偶联得多肽与O型FMDV阳性血清的结合能力,结果显示,中和表位Pep1、Pep2、Pep3能特异性结合O型FMDV感染血清和免疫血清,为抗FMDV中和表位单克隆抗体的制备和FMDV抗体检测试纸条方法的建立奠定基础。
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| 关 键 词： | FMDV 抗原表位 多肽 合成 鉴定 |
Synthesis and Identification of Key Protective Antigen Peptides of Foot-and-mouth Disease Virus |
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| YANG Suzhen,ZHAO Dong,ZHI Yubao,DENG Ruiguang and ZHANG Gaiping. Synthesis and Identification of Key Protective Antigen Peptides of Foot-and-mouth Disease Virus[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2014, 23(10): 8-12 |
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| Authors: | YANG Suzhen ZHAO Dong ZHI Yubao DENG Ruiguang ZHANG Gaiping |
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| Abstract: | To screening epitope antigen of FMDV, five FMDV serotype O specific epitope peptides were synthesized by 12-Symphony Quartet peptide synthesizer according to Fmoc solid phase synthesize principle. Molecular characteristics analysized by Quattro Micro LC-MS indicated that the 5 peptides were synthesized successfully. Peptide-BSA conjugates were analyzed by Dot-blot and indirect ELISA, and the results showed that Pep1, Pep2 and Pep3 can bind to both FMDV type O infected sera and immunized sera specifically. It provides materials for the development of immunochromatographic strip for the detection of FMDV antibody and the preparation of FMDV monoclonal antibody. |
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| Keywords: | FMDV Antigenic epitope Peptide Synthesis Identification |
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