| 疣粒野生稻半胱氨酸蛋白酶基因ME085的cDNA全长克隆及序列分析 |
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| 引用本文： | 陈 良,李定琴,钟巧芳,殷富有,付 坚,罗红梅,程在全.疣粒野生稻半胱氨酸蛋白酶基因ME085的cDNA全长克隆及序列分析[J].西北农业学报,2014,23(4):22-27. |
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| 作者姓名： | 陈 良 李定琴 钟巧芳 殷富有 付 坚 罗红梅 程在全 |
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| 作者单位： | ( 1.云南农业大学 农学与生物技术学院，昆明 650000; 2.云南省农业科学院 生物技术与种质资源研究所，昆明 650000; 3.云南省农业生物技术重点实验室，昆明 650000 ; 4.云南昆明农业部西南作物资源与新种质创制重点实验室，昆明 650000) |
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| 基金项目： | 国家自然科学基金(31000746，31160067)；公益性行业专项(201003021)；云南省科技项目（2010CD098，2012CH010）；云南高端人才引进项目（2010CI120）。 |
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| 摘 要： | 为了发掘疣粒野生稻抗性基因,从受白叶枯菌诱导的疣粒野生稻叶片抑制差减文库(SSH文库)中选择抗病相关的EST序列,运用cDNA末端快速扩增法(RACE)获得一个基因全长,命名为ME085。通过生物信息学分析表明该基因全长1 171bp,具有一个750bp的开放阅读框,编码249个氨基酸,其理论等电点为5.76,相对分子质量为27 580.1,存在一个明显的半胱氨酸蛋白酶结构域。推测该基因是一个半胱氨酸蛋白酶基因。
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| 关 键 词： | 疣粒野生稻 RACE 半胱氨酸蛋白酶 cDNA克隆 序列分析 |
Cloning and Sequence Analysis on Full-length cDNA of Cysteine Protease Gene ME085 from Oryza meyeriana |
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| CHEN Liang,LI Dingqin,ZHONG Qiaofang,YIN Fuyou,FU Jian,LUO Hongmei and CHENG Zaiquan.Cloning and Sequence Analysis on Full-length cDNA of Cysteine Protease Gene ME085 from Oryza meyeriana[J].Acta Agriculturae Boreali-occidentalis Sinica,2014,23(4):22-27. |
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| Authors: | CHEN Liang LI Dingqin ZHONG Qiaofang YIN Fuyou FU Jian LUO Hongmei and CHENG Zaiquan |
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| Abstract: | In order to finding the resistance genes of Oryza meyeriana, the EST related to disease resistance was selected from subtraction cDNA library of Oryza meyeriana induced by Xanthomonas campestris pv.oryzae. Based on the EST, we obtained a full length of cDNA and named ME085 by rapidly amplification cDNA ends. Bioinformatic analysis showed the gene sequence was 1 171 bp including 750 bp of open reading frame(ORF) which encoding a protein of 249 amino acids with a predicted relative molecular mass of 27 580.1 and a hypothetical pI(isoelectric point) of 5.76. Meanwhile, there is an obvious domain of Cysteine protease. Therefore, ME085 is deduced as a Cysteine protease gene. |
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| Keywords: | Oryza meyeriana RACE Cysteine protease cDNA clone Sequence analysis |
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