| 美洲型猪蓝耳病病毒高致病性变异株RT-LAMP检测方法的建立 |
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| 引用本文： | 曾少灵,孙洁,秦智锋,阮周曦,林庆燕,花群义,曹琛福,杨俊兴,吕建强,张彩虹,陈兵,陈书琨. 美洲型猪蓝耳病病毒高致病性变异株RT-LAMP检测方法的建立[J]. 动物医学进展, 2010, 31(12) |
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| 作者姓名： | 曾少灵 孙洁 秦智锋 阮周曦 林庆燕 花群义 曹琛福 杨俊兴 吕建强 张彩虹 陈兵 陈书琨 |
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| 作者单位： | 深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001;深圳出入境检验检疫局动植中心,深圳市外来有害生物检测技术研发重点实验室,广东深圳,518001 |
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| 摘 要： | 设计两套反转录-环介导等温扩增(RT-LAMP)引物,建立了检测美洲型猪蓝耳病病毒及其高致病性变异株的RT-LAMP方法。采用RT-LAMP方法与实时荧光RT-PCR方法检测58份病料,结果一致,但RT-LAMP方法的灵敏度大约高出10倍。RT-LAMP方法特异性良好,检测猪瘟、伪狂犬病和细小病毒等均未出现交叉反应。结果表明,建立的美洲型猪蓝耳病病毒及其高致病性变异株RT-LAMP检测方法具有特异、灵敏、高效和简便的特点,在我国猪蓝耳病快速检测领域将有广阔的应用前景。
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| 关 键 词： | RT-LAMP 猪蓝耳病 猪蓝耳病病毒高致病性变异株 |
Development of RT-LAMP Test for Highly Pathogenic American Genotype PRRSV Mutation Strains |
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| ZENG Shao-ling,SUN Jie,QIN Zhi-feng,RUAN Zhou-xi,LIN Qing-yan,HUA Qun-yi,CAO Chen-fu,YANG Jun-xing,L Jian-qiang,ZHANG Cai-hong,CHEN Bing,CHEN Shu-kun. Development of RT-LAMP Test for Highly Pathogenic American Genotype PRRSV Mutation Strains[J]. Progress In Veterinary Medicine, 2010, 31(12) |
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| Authors: | ZENG Shao-ling SUN Jie QIN Zhi-feng RUAN Zhou-xi LIN Qing-yan HUA Qun-yi CAO Chen-fu YANG Jun-xing L Jian-qiang ZHANG Cai-hong CHEN Bing CHEN Shu-kun |
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| Affiliation: | ZENG Shao-ling,SUN Jie,QIN Zhi-feng,RUAN Zhou-xi,LIN Qing-yan,HUA Qun-yi,CAO Chen-fu,YANG Jun-xing,L Jian-qiang,ZHANG Cai-hong,CHEN Bing,CHEN Shu-kun(Animals and Plants Inspection and Quarantine Institute,Shenzhen Entry-Exit Inspection & Quarantine Bureau,Shenzhen Key Laboratory of Inspection Research & Development of Alien Pests,Shenzhen,Guangdong,518001,China) |
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| Abstract: | In this study,RT-LAMP test methods for the American genotype PRRSV and its highly pathogenic mutation strains were set up with two sets of specific primers.Test results of 58 samples detected by RT-LAMP and real-time RT-PCR respectively showed in accordance,but the developed RT-LAMP methods were at least 10 times more sensitive than RT-PCR method.There was no cross-reaction between PRRSV and the other kinds of viruses,such as CSFV,PRV and PPV in the RT-LAMP tests.The two developed RT-LAMP methods here are s... |
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| Keywords: | RT-LAMP PRRS highly pathogenic American PRRSV mutation strain |
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