蝴蝶兰花梗腋芽离体快繁控制褐变的研究

摘要：褐变是蝴蝶兰离体培养中的常见现象，本试验尝试探寻蝴蝶兰花梗腋芽离体快繁过程中有效控制其褐变的方法，诱导丛生芽。试验证明：带腋芽花梗节段褐变程度比腋芽要轻；适当的暗培养有利于减轻外植体的褐变；细胞分裂素KT比BA的褐变症状要轻，但丛生芽的诱导率不如BA；适当添加一定剂量的PVP、活性炭、柠檬酸等可减轻褐变，柠檬酸、PVP效果比活性炭显著，为特色花卉的工厂化生产提供参考。

Study of rapid propagation axillary browning control about Butterfly orchids stems in vitro

Abstract:Browning is a common phenomenon of Phalaenopsis in vitro culture, this test attempts to explore the rapid propagation effective way of butterfly orchid stems Axillary process of in vitro to control their browning, bud induction. Tests prove that all those with axillary pedicel browning segment are lighter than axillary and Cytokinin KT browning symptoms lighter than BA, Bud induction rate than BA; It may reduce the browning that add the appropriate dose of PVP, activated carbon, citric acid etc, citric acid and PVP significantly make better than activated carbon, which provides a reference for the factory about the production of Characteristics flowers and plants.