黑松13个抗病家系对松材线虫的抗性反应及组织病理学观察

 为评估自日本引进的抗性黑松对松材线虫(Pine Wood Nematode,PWN)的抗病性,选用3个不同来源的PWN强毒和中强毒虫株采用皮接法接种,对1.5年生的黑松13个优良家系进行抗病性测定。结果表明,引进的黑松大部分家系对3个PWN虫株都表现出一定的抗病性,但针对不同虫株,抗病各家系间差异明显,其中有7个家系(3、4、5、6、9、11和12号)表现良好。同时,为探讨引进黑松家系感染PWN后的组织病理反应,对12号抗性家系进行组织病理学研究。观察表明,松材线虫在抗性黑松和普通黑松中的纵向扩散速度差别不明显,但其在抗性黑松内的横向扩散速度比在普通黑松内要慢,且抗性黑松体内木射线细胞和围绕树脂道泌脂细胞的薄壁细胞,出现病变要比普通黑松晚;在接种后同一时间取茎段的同一部位发现,抗性松苗组织解剖结构及细胞的病变和破坏程度要比普通松苗轻。

Resistance and histopathological observation of wilt-resistant Pinus thunbergii families from Japan to Bursaphelenchus xylophilus

For determing the resistance of 13 wilt-resistant black pine(Pinus thunbergii) families to Bursaphe-lenchus xylophilusa introduced from Japan,3 isolates of B.xylophilus were inoculated into the stems of 18-months old pines with bark inoculation.The resistance and histopathological reaction to nematode infection of these families were investigated.The results showed that most of 13 families were resistant,but there were significant differences in terms of the resistance to different nematode isolates.No.3,4,5,6,9,11 and 12 of these pine families had better resistant effects.The histopathological observation of family No.12 indicated that immigrating rate of the pine wood nematodes in the pine seedlings was as fast as in the controls vertically,but slower than in the control pine seedlings horizontally.In addition,pathological changes in parenchyma radial cells and parenchymarous cells around resinosis cells in wilt-resistant pine seedlings occurred later than in the control.Sampling the same stem segment at the same time,it showed that the pathological changes occurred in wilt-resistant pine seedlings more gently than in the controls.