| 狼尾蕨体细胞耐盐突变体NY-7的耐盐性研究 |
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| 引用本文： | 梁丽建,叶晓青,贾新平,孙晓波,佘建明,汤日圣,邓衍明. 狼尾蕨体细胞耐盐突变体NY-7的耐盐性研究[J]. 江西农业学报, 2013, 0(11): 15-18,21 |
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| 作者姓名： | 梁丽建 叶晓青 贾新平 孙晓波 佘建明 汤日圣 邓衍明 |
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| 作者单位： | 江苏省农业科学院农业生物技术研究所、江苏省农业生物学重点实验室,江苏南京210014 |
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| 基金项目： | 江苏省农业科技自主创新基金项目[CX（13）5057]. |
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| 摘 要： | 通过盆栽实验，研究了不同盐浓度胁迫下狼尾蕨及其体细胞耐盐突变体NY-7的5个生理指标，并应用模糊隶属函数法对其耐盐性进行了综合评价。结果显示：随着NaCI浓度的升高和胁迫时间的延长，NY-7的叶绿素和可溶性蛋白含量下降，脯氨酸含量、SOD活性均没有显著变化，MDA含量有所增加；而狼尾蕨的叶绿素含量显著降低，SOD活性、脯氨酸、可溶性蛋白和MDA含量均显著增加；NY-7的耐盐性高于狼尾蕨。
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| 关 键 词： | 狼尾蕨 体细胞突变体 耐盐性 综合评价 |
Research on Salt Tolerance of Salt- tolerant Somatic Cell Mutant NY -7 from Davallia bullata |
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| Affiliation: | LIANG Li - jian, YE Xiao - qing, JIA Xin - ping, SUN Xiao - bo, SHE Jian - ming, TANG Ri - sheng, DENG Yan - ming ( Institute of Agro - biotechnology, Jiangsu Academy of Agricultural Sciences ; Jia ,su Provincial Key Laboratory of Agrobiology, Nanjing 210014, China) |
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| Abstract: | Abstract ： Using Davallia bullata and its salt - tolerant somatic cell mutant NY - 7 as experimental materials, we studied their 5 physiological indexes under different salt concentrations stress through pot experiments, and comprehensively evaluated their salt tolerance by using fuzzy subordinate function method. The results showed that： when salt concentration increased and stress time extended, the contents of both leaf chlorophyll and soluble protein showed the trend of declining in salt - tolerant mutant NY - 7, while its proline content and SOD activity did not present significant changes, and its content of MDA increased slightly. In comparison with NY - 7, the leaf chlorophyll content of Davallia bullata significant decreased, and its SOD activity and the contents of proline, soluble protein and MDA significantly increased. The comprehensive evaluation of five physiological indexes suggested that the salt tolerance of NY - 7 was higher than that of Davallia buUata. |
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| Keywords: | Davallia bullata Somatic cell mutant Salt tolerance Comprehensive evaluation |
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