都百凤桃树离体植株再生培养的研究

以从日本引进的桃树品种都百凤为试材，采用正交设计，研究了6-BA，NAA，接种芽数，活性炭对不定芽增殖与壮苗的影响，探讨了激素、培养基盐浓度、活性炭对都百凤组培苗生根的影响。结果表明，都百风桃树离体植株再生继代增殖培养基为MS＋6BA3mg／L～5mg／L＋NAA0．1mg／L～0．2mg／L＋蔗糖30g／L，培养基pH值为5.9，以3芽为1个转接单位，培养温度为25℃，光照强度2000Lx，光照时间16h／d；生根培养基为1／2MS＋IBA0.2mg／L＋蔗糖20g／L＋琼脂7g／L＋活性炭0．4g／L，培养基pH值为6．0，培养温度为22℃，光照强度1600Lx，光照时间16h／d；组培苗转入生根培养基舌暗处理2d，再转到光照下培养有利于生根。

Researches on Regeneration Culture to Excised Plant of Dubaifeng Peach

Taking Dubaifeng peach as material, the effect of 6-BA, NAA, inocutation bud number, active carbon on proliferation of adventitious bud and on making seedling strong were researched, of hormone, salt concentration of culture medium, active carbon on rooting of tissue culture seedling were inquired by orthogonal design. The resuits shows that the regeneration bud of excised plant of Dubaifeng peach should be transferred to the medium of MS＋6-BA 3 mg/L-5 mg/L＋NAA 0.1 mg/L-0.2 mg/L ＋ cane sugar 30 g/L, pH 5.9, temperature 25℃, light intensity 2 000 Lx, light period 16 h/d, and 3 buds as a transferring unit; The tissue culture seedling should be transferred to the rooting medium of 1/2 MS-IBA 0.2 mg/L ＋ cane sugar 20 g/L ＋ agars 7 g/L ＋ activated carbon 0.4 g/L, pH 6.0, temperature 22℃, light intensity 1 600 Lx, light period 16 h/d; The tissue culture seeding was transferred to rooting culture medium to cultivate for 2 days without light and then was cultivated in light, which helps to root.