| 一个与西瓜核不育育性基因连锁的AFLP分子标记的获得 |
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| 引用本文： | 刘海河,张彦萍,郭守鹏,谢彬,张成合. 一个与西瓜核不育育性基因连锁的AFLP分子标记的获得[J]. 河北农业大学学报, 2009, 32(1) |
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| 作者姓名： | 刘海河 张彦萍 郭守鹏 谢彬 张成合 |
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| 作者单位： | 河北农业大学,园艺学院,河北,保定,071001;河北工程大学,农学院,河北,邯郸,056038;河北工程大学,农学院,河北,邯郸,056038;河北农业大学,园艺学院,河北,保定,071001 |
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| 基金项目： | 河北省自然科学基金，河北农业大学博士科研基金 |
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| 摘 要： | 以西瓜G17AB核雄性不育两用系为材料,采用BAS法构建了不育池和可育池。利用AFLP分子标记技术,通过筛选256对AFLP引物组合,获得了一个与育性位点紧密连锁的AFLP标记,片段长度在310~350 bp之间,该标记与育性基因的遗传距离为5.0 cM。
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| 关 键 词： | 西瓜 核雄性不育基因 AFLP标记 |
A AFLP marker linked to male fertile gene in G17AB male sterile line of watermelon |
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| LIU Hai-he,ZHANG Yan-ping,GUO Shou-peng,XIE Bin,ZHANG Oheng-he. A AFLP marker linked to male fertile gene in G17AB male sterile line of watermelon[J]. Journal of Agricultural University of Hebei, 2009, 32(1) |
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| Authors: | LIU Hai-he ZHANG Yan-ping GUO Shou-peng XIE Bin ZHANG Oheng-he |
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| Affiliation: | 1.College of Horticure;Agricultural University of Hebei;Baoding 071001;China;2.College of Agronomy;Hebei Engineering University;Handan 056038;China |
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| Abstract: | Molecular maker linked to the male fertile gene in G17AB genic male sterile line of watermelon was screened with BSA method and AFLP technique.A specific polymorphism fragment between male sterile bulk and male fertile bulk was obtained with E31M50 primer pair.Its length is between 310 bp and 350 bp,and the heredity distance to the male fertility gene is about 5.0 cM. |
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| Keywords: | watermelon male-sterility gene AFLP marker |
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