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Molecular cytogenetics in hop (Humulus lupulus L.) and identification of sex chromosomes by DAPI-banding |
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| Authors: | G.I. Karlov T.V. Danilova C. Horlemann G. Weber |
| Affiliation: | (1) Department of Biotechnology, Timiryazev Agricultural Academy, Timiryazevskaya st. 44, 127550 Moscow, Russia;(2) Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Department of Plant Breeding and Biotechnology, D-70593 Stuttgart, Germany |
| Abstract: | Hop (Humulus lupulus L.) waskaryotyped by Fluorescence In SituHybridization (FISH) with probes 18S-25Sand 5S rDNA as well as DAPI banding andmeasurements of lengths of chromosome.Irrespective of the sex of the plants theseprobes revealed one signal near thetelomere of chromosome 6 and two signalseach on chromosomes 2 and 5.The shortest chromosome in a metaphaseplate of a male plant was designated to bechromosome Y. It displayed neither signalswith FISH nor DAPI bands. A middle sizedmetacentric chromosome with no telomericbut with an interstitial DAPI band on theshort arm was designated as X. Allautosomes displayed telomeric DAPI-positivebands. For the first time all mitotic hopchromosomes including the sex chromosomeswere identified by methods of molecularcytogenetics. |
| Keywords: | fluorescence in situ hybridization hop Humulus lupulus (L.) karyotype sex chromosomes rDNA |
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