| 二棱大麦LEA cDNA的克隆与测序 |
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| 引用本文： | 郭卫东,饶景萍,李嘉瑞. 二棱大麦LEA cDNA的克隆与测序[J]. 西北农林科技大学学报(自然科学版), 2000, 28(2): 8-12 |
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| 作者姓名： | 郭卫东 饶景萍 李嘉瑞 |
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| 作者单位： | 1. 西北农林科技大学园艺系,陕西杨陵,712100
2. 中国热带农业科学院生物技术国家重点实验室,海南儋州,571737 |
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| 基金项目： | 杨陵农业科技开发基金资助项目! ( 41 3 0 2 0 2 ) |
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| 摘 要： | 从二棱大麦 ( H ordeum distichon Linn.)幼苗中提取总 RNA,通过 RT- PCR获得目的片段 HDCS1 ,并采用 T-载体进行克隆。测序结果表明 ,HDCS1全长 664bp,含有 1个完整的阅读框架。其编码蛋白质全长 2 0 2个氨基酸 ,含有 8个由 1 1个氨基酸残基组成的基元序列 ,属第 3组 LEA蛋白。HDCS1与大麦 ( H ordeum vulgare L.) L EA蛋白、HVA1具有高度同源性 ,其差异在于 HDCS1比 HVA1缺少一个完整的由 1 1个氨基酸残基组成的基元序列
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| 关 键 词： | 二棱大麦 LEA基因 克隆 |
Cloning of a group 3 Lea cDNA from tworow barley |
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| RAO Jing-ping. Cloning of a group 3 Lea cDNA from tworow barley[J]. Journal of Northwest A&F University（Natural Science Edition）, 2000, 28(2): 8-12 |
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| Authors: | RAO Jing-ping |
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| Affiliation: | 2 (1 Department of Horticulture,Northwest Science and Technology University of Agricultural and Forestry,Yangling,Shaanxi 712100,China) (2 National Key Biotechnology Laboratory for Tropi |
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| Abstract: | Total RNA is extracted from tworow barley ( Hordeum distichon Linn.) seedlings induced with 0.1 mmol/L ABA and reversely transcribed to first strands of cDNA.The interest fragment,HDCS1,is obtained by PCR and cloned by T overhang vectors.HDCS1 is 664 bp long containing one open reading frame.It encodes a deduced protein of 202 amino acid residues,which contains eight motifs composed of 11 amino acids and belongs to group 3 LEA protein.HDCS1 is highly homologous to barley ( H.vulgare Linn.) HVA1.The only difference between them is that the number of 11 mer repeating units of HDCS1 is one less than that of HVA1. |
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| Keywords: | tworow barley LEA cDNA cloning |
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