| 用PCR-RFLP技术鉴别猪的氟烷基因型 |
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| 引用本文： | 郭雁君,罗安怡,陈继华. 用PCR-RFLP技术鉴别猪的氟烷基因型[J]. 四川农业大学学报, 1999, 17(1): 54-57 |
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| 作者姓名： | 郭雁君 罗安怡 陈继华 |
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| 作者单位： | [1]四川农业大学动物科技学院 [2]乐山市种畜场 |
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| 基金项目： | 四川省“九五”攻关项目 |
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| 摘 要： | 用ＰＣＲ－ＲＦＬＰ技术对乐山市种畜场长白猪选育基础群的氟烷基因型进行了检测，查清了Ｈａｌｎ基因在该群体中的分布，Ｈａｌｎ基因的频率为１８０５６％。同时对影响ＰＣＲ的几个主要因素进行了初步研究。
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| 关 键 词： | 猪 PCR－RFLP技术 氟烷基因 |
IDENTIFICATION ON PORCINE HALOTHANE GENOTYPES BY POLYMEROSE CHAIN REACTION |
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| Guo Yanjun Luo Anzhi et al. IDENTIFICATION ON PORCINE HALOTHANE GENOTYPES BY POLYMEROSE CHAIN REACTION[J]. Journal of Sichuan Agricultural University, 1999, 17(1): 54-57 |
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| Authors: | Guo Yanjun Luo Anzhi et al |
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| Abstract: | Porcine Stress Syndrome(PSS) is controlled by Halothanegene located on porcine No.6 chromosome. Identification of the porcineHalothane genotypes was established by PCR-RFLP. The Halothane genefragment containing the mutation point was amplified and the PCR product was incubated with restiction endonuclease HhaI, then genotypes were detected. And several factors that effected PCR are analysised. |
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| Keywords: | PORCINE STRESS SYNDROME HALOTHANE GENOTYPE POLYMERASECHAIN REACTION (PCR). |
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