E.tenella吉林株单卵囊的分离及PCR鉴定

[目的]为了获得E．tenella纯株，进行相关的分子生物学研究。[方法]对已经建立的球虫单卵囊分离技术进行改进，用分离的单卵囊胶囊接种雏鸡。同时应用PCR方法对收集的单卵囊分离株进行球虫种类鉴定。[结果]20只试验鸡中有15只粪便中分离到卵囊，感染成功率为75％。PCR扩增结果表明该单卵囊分离株为Etenella。[结论]采用单卵囊胶囊进行接种，操作简便，既节省了接种时间，又降低了接种难度，且大大提高了接种成功率，为球虫的分子生物学研究提供了材料。

Isolation of E.tenella JL Strain Single-oocyst and PCR Identification

[Objective] In order to obtain a purified strain of E.tenella, relative molecular biology researches were carried out.[Method] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens.At the same time, the collected oocysts were identified by PCR method.[Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%.The PCR results demonstrated that the single-oocyst strain was E.tenella.[Conclusion] The inoculation of single-oocyst capsule was simple, besides, this method did not only save time but also declined inoculation difficulty ,increased infection rate and provided good materials for biological research of coccidia.