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E.tenella吉林株单卵囊的分离及PCR鉴定
引用本文:杨桂连,李建华,张西臣,赵权,宫鹏涛,蔡亚南,张国才.E.tenella吉林株单卵囊的分离及PCR鉴定[J].安徽农业科学,2009,35(19):8893-8894.
作者姓名:杨桂连  李建华  张西臣  赵权  宫鹏涛  蔡亚南  张国才
作者单位:吉林大学畜牧兽医学院,吉林长春130062;吉林农业大学动物科学技术学院,吉林,长春130118;吉林大学畜牧兽医学院,吉林长春,130062
摘    要:目的]为了获得E.tenella纯株,进行相关的分子生物学研究。方法]对已经建立的球虫单卵囊分离技术进行改进,用分离的单卵囊胶囊接种雏鸡。同时应用PCR方法对收集的单卵囊分离株进行球虫种类鉴定。结果]20只试验鸡中有15只粪便中分离到卵囊,感染成功率为75%。PCR扩增结果表明该单卵囊分离株为Etenella。结论]采用单卵囊胶囊进行接种,操作简便,既节省了接种时间,又降低了接种难度,且大大提高了接种成功率,为球虫的分子生物学研究提供了材料。

关 键 词:E.tenella  单卵囊  PCR鉴定

Isolation of E.tenella JL Strain Single-oocyst and PCR Identification
Institution:YANG Gui-lian et al (College of Animal Science and Veterinary Medicine, Jilin University, Changchun,Jilin 130062)
Abstract:Objective] In order to obtain a purified strain of E.tenella, relative molecular biology researches were carried out.Method] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens.At the same time, the collected oocysts were identified by PCR method.Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%.The PCR results demonstrated that the single-oocyst strain was E.tenella.Conclusion] The inoculation of single-oocyst capsule was simple, besides, this method did not only save time but also declined inoculation difficulty ,increased infection rate and provided good materials for biological research of coccidia.
Keywords:E  tenella  E  tenella  Single-oocyst  PCR identification
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