| 鳜野生群体与养殖群体的RAPD分析 |
| |
| 引用本文： | 方展强,陈军,郑文彪,伍育源,肖智.鳜野生群体与养殖群体的RAPD分析[J].大连水产学院学报,2005,20(1):16-19. |
| |
| 作者姓名： | 方展强 陈军 郑文彪 伍育源 肖智 |
| |
| 作者单位： | 华南师范大学,生命科学学院,广东,广州,510631 |
| |
| 基金项目： | 广东省科技厅科技攻关项目 |
| |
| 摘 要： | 用60个随机引物对鳜Siniperca chuatsi Basilewsky野生群体和养殖群体进行随机扩增多态DNA(RAPD)分析，经过引物筛选，分别用55个和54个引物对野生鳜和养殖鳜进行群体内分析。结果表明，野生群体多态位百分率为85．75％，群体内遗传相似率和遗传距离分别为0．8547和0．1453；养殖群体多态位百分率为16．39％，遗传相似率和遗传距离分别为0．9527和0．0473；两群体间遗传相似率和遗传距离分别为0．6905和0．3095；鳜野生群体具有较高的遗传多样性，养殖群体的遗传多样性却显著降低，野生群体与养殖群体间有明显的遗传差异，说明人工繁育对鳜基因组造成了显著的影响。
|
| 关 键 词： | 鳜 野生群体 养殖群体 随机扩增多态 DNA |
| 文章编号： | 1000-9957(2005)01-0016-04 |
RAPD analysis of wild population and cultivated population in Siniperca chuatsi Basilewsky |
| |
| FANG Zhan-qiang,CHEN Jun,ZHENG Wen-biao,WU Yu-yuan,XIAO Zhi.RAPD analysis of wild population and cultivated population in Siniperca chuatsi Basilewsky[J].Journal of Dalian Fisheries University,2005,20(1):16-19. |
| |
| Authors: | FANG Zhan-qiang CHEN Jun ZHENG Wen-biao WU Yu-yuan XIAO Zhi |
| |
| Abstract: | Cultivated and wild populations of mandarinfish (Siniperca chuatsi Basilewsky) were analyzed using 60 random primers by randomly-amplified polymorphic DNA (RAPD) method. The results indicated that the percentage of polymorphic sites, the genetic similarity rate and the genetic distance in wild population and cultivated population of the mandarinfish were 85.75% and 16.39%, 0.8547 and 0.9527, 0.1453 and (0.0473), respectively. The low genetic similarity rate(0.6905) and high genetic distance(0.3095) between two populations indicated that there was a significantly genetic differentiation, suggesting that aquaculture had led to decrease in genetic diversity. |
| |
| Keywords: | Siniperca chuatsi cultivated population wild population randomly amplified polymorphic DNA (RAPD) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
