| 葡萄扇叶病毒RT-PCR检测技术研究 |
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| 引用本文： | 牛建新,鲁晓燕,陈萍. 葡萄扇叶病毒RT-PCR检测技术研究[J]. 西北农业学报, 2003, 12(3): 81-85 |
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| 作者姓名： | 牛建新 鲁晓燕 陈萍 |
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| 作者单位： | 石河子大学农学院园林系,石河子,832003 |
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| 基金项目： | 农业部"九五"课题(编号:垦-06-24);兵团科委资助项目(NKB02SDXNK01SW). |
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| 摘 要： | 以扇叶病株为材料,根据病毒编码外壳蛋白的核苷酸序列,设计特异引物P_1(1,064—1,083),P_2(762—781),建立了以RNA为模板的GFLV的RT-PCR检测体系。PCR产物电泳谱带明显,与预期的引物应扩增321 bp片段完全吻合。并利用建立起来的技术体系,对田间葡萄进行了随机取样检测,取得了很好效果。此方法操作简单,检测周期短,准确率高,具有广泛和更进一步的应用价值。
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| 关 键 词： | 葡萄扇叶病毒 RT-PCR RNA 检测 |
| 文章编号： | 1004-1389(2003)03-0081-05 |
| 收稿时间： | 2002-12-06 |
| 修稿时间： | 2002-12-06 |
Studies on RT-PCR Detection technology of Grapevine Fan Leaf Virus |
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| NIU Jian-xin,LU Xiao-yan and CHEN Ping. Studies on RT-PCR Detection technology of Grapevine Fan Leaf Virus[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2003, 12(3): 81-85 |
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| Authors: | NIU Jian-xin LU Xiao-yan CHEN Ping |
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| Affiliation: | Department of Horticulture and Forestry, Agriculture College of Shihezi University, Shihezi 832003, China;Department of Horticulture and Forestry, Agriculture College of Shihezi University, Shihezi 832003, China;Department of Horticulture and Forestry, Agriculture College of Shihezi University, Shihezi 832003, China |
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| Abstract: | RT-PCR primers were designed based on the GFLV coat protein region, Located at the 3' end of RNA2. Primer 1 is complementary to nucletides 1,064-1,083," Primer 2 corresponds to nu-cleotides 762-781. Preliminary RT-PCR experiment with RNA showed that these primers are suffi cient to amply DNA of the expected size(321 bp). In virtue of easy-manufacture,short-period,high-accuracy , think of the potential value on further application, RT-PCR diagnosis has important value of theory and practice. It will give great impetus on virus-free manufacture. |
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| Keywords: | Grapevine Fan leaf virus RT-PCR RNA Diagnosis |
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