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| 小麦远缘杂交后代花药培养最佳条件的研究 | |
| 引用本文： | 朱艳,汤剑力,吴根法,潘重光,曹清玉,沈大棱.小麦远缘杂交后代花药培养最佳条件的研究[J].上海交通大学学报(农业科学版),2000,18(2):138-141. |
| 作者姓名： | 朱艳 汤剑力 吴根法 潘重光 曹清玉 沈大棱 |
| 作者单位： | 1. 上海宝山区生物技术中心,上海,201907 2. 上海交通大学植物科学系,上海,201101 3. 复旦大学,遗传所,上海,200433 |
| 基金项目： | 上海市科委科学基金资助项目(993113027) |
| 摘 要： | 对远缘杂交后代花药培养条件的比较研究发现w14培养基比C47培养基更适用于小麦与小黑麦杂交后代的花药培养。花药培养的出愈率、再生率和绿苗率均和材料的遗传基础有一定的关系。培养中预处理温度和时间、初培养温度条件、花药密度效应、壮苗培养以及安全越夏都是提高花药培养效率的必要条件。 |
| 关 键 词： | 小麦 小黑麦 花药培养 远缘杂交 |
| 文章编号： | 1000-193X(2000)02-0138-04 |
| 修稿时间： | 2000-04-07 |
Studies on anther culture of outbreeding progenies from triticum X triticale |
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| ZHU Yon,TANG Jian-li,WU Geng-f,PAN Chong-guang,CAO Qing-yu,SHEN Da-ling.Studies on anther culture of outbreeding progenies from triticum X triticale[J].Journal of Shanghai Jiaotong University (Agricultural Science),2000,18(2):138-141. | |
| Authors: | ZHU Yon TANG Jian-li WU Geng-f PAN Chong-guang CAO Qing-yu SHEN Da-ling |
| Institution: | ZHU Yon ,TANG Jian-li ,WU Geng-fa ;(Shanghia Baoshan Bietechnulogical Center, Shanghai 201907. China);PAN Chong-guang ;(Dept. Of Plant Science, Shanghai Jiaotong Univ.,Shanghai 201101, China);CAO Qing-yua ,SHEN Da-ling ;(Institute of Genetics, Fudan Univ.,Shanghai 200433, China) |
| Abstract: | Anther culture of outbreeding progenies from tfiticum X triticale was carried out on W14 and C17, medium. The data showed that W14 medium was more suitable to the anther culture than C17 medium. The ability to induce callus from anthers, plant regeneration and green plant regenerated was found to be species-or genotype-dependent. The essefial conditions for anther culture capacity are. Pretreatment temperature and time, culture temperature, anther density, strong seedling curare and safely ovemummering. |
| Keywords: | triticum trillcale anther culture outbreeding |
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